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Cell-Free Gene Expression from DNA Brushes

2022
Linear double-stranded DNA polymers coding for synthetic genes immobilized on a surface form a brush as a center for cell-free gene expression, with DNA density 102-103 fold higher than in bulk solution reactions. A brush localizes the transcription-translation machinery in cell extracts or in cell-free reconstituted reactions from purified components,
Michael, Levy   +6 more
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Photopatterned Polymer Brushes Promoting Cell Adhesion Gradients

Langmuir, 2006
The ability to spatially control cellular adhesion in a continuous manner on a biocompatible substrate is an important factor in designing new biomaterials for use in wound healing and tissue engineering applications. In this work, a novel method of engineering cell-adhesive RGD-ligand density gradients to control specific cell adhesion across a ...
Bradley P, Harris   +5 more
openaire   +2 more sources

Brush cells in the human fetal trachea

Pediatric Pulmonology, 1990
AbstractWe performed a morphologic examination of human fetal lung tissue, using scanning and transmission electron microscopy, in order to establish the presence of brush cells in extrapulmonary and intrapulmonary airways, and developing acinar epithelium.
M F, DiMaio   +4 more
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Postnatal differentiation of unipolar brush cells and mossy fiber-unipolar brush cell synapses in rat cerebellum

Neuroscience, 2001
The unipolar brush cells are excitatory, cerebellar granular layer interneurons that receive mossy fiber input on their dendritic brushes in the form of a giant glutamatergic synapse. We investigated the postnatal development of the brush of the unipolar brush cell in lobules IX and X by light microscopy and defined the maturation of mossy fiber ...
F, Morin, M R, Diño, E, Mugnaini
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Quantitative distribution of brush cells in the rat gastrointestinal tract: brush cell population coincides with NaHCO3 secretion

Medical Molecular Morphology, 2011
The function of brush cells is obscure, but recent cytochemical studies indicate that rat bile duct brush cells secrete NaHCO(3). The aim of this study was to determine the quantitative distribution of brush cells at 16 sites of the rat gastrointestinal tract and to investigate the role of NaHCO(3) secretion at these sites. Specimens of 16 sites of the
Toyokazu, Akimori   +5 more
openaire   +2 more sources

Purkinje cell phenotype restricts the distribution of unipolar brush cells

Neuroscience, 2009
Cerebellar unipolar brush cells (UBCs) are glutamatergic interneurons of the granular layer. Previous studies have identified three distinct UBC subsets in the mouse cerebellar cortex: one expressing the calcium-binding protein calretinin (CR), a second expressing both the metabotropic glutamate receptor (mGluR)1alpha and phospholipase C(PLC)beta4, and
CHUNG SH   +5 more
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Presence of brush cells in the mouse gallbladder

Microscopy Research and Technique, 1997
The brush cells (BC) are the second most frequent cellular component of the epithelium of the mouse gallbladder. They have a topographical distribution, being present in large numbers toward the neck and in the fundic regions of the organ and are scattered in the body. Serial section studies demonstrate that BC have a characteristic shape consisting of
L, Luciano, E, Reale
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Unipolar Brush Cells

2016
The unipolar brush cells (UBCs) are excitatory interneurons in the granule cell layer of the cerebellar cortex. They amplify extra-cerebellar inputs from vestibular origin as well as other inputs whose origin is the focus of ongoing research. The UBCs are classified in two functionally and chemically distinct subclasses. Type I UBCs express calretinin,
Marco Martina, Gabriella Sekerková
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