Results 51 to 60 of about 247,217 (307)

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Responses of Emergent Marsh Wetlands in Upstate New York to Variations in Urban Typology

Ecology and Society, 2004
Although it has been repeatedly demonstrated that urbanization has negative environmental consequences, the conversion of land to urban use is increasing worldwide and is not likely to abate. We tested the hypothesis that different urban typologies, i.e.,
G. S. Kleppel, Shirley A. Madewell, Sarah E. Hazzard   +2 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Detrimental effect of zwitterionic buffers on lysosomal homeostasis in cell lines and iPSC-derived neurons [version 1; peer review: 3 approved]

AMRC Open Research, 2020
Good’s buffers are commonly used for cell culture and, although developed to have minimal to no biological impact, they cause alterations in cellular processes such as autophagy and lysosomal enzyme activity. Using Chinese hamster ovary cells and induced
Nicholas D. Allen, Helen Waller-Evans, Emyr Lloyd-Evans, Rafael A. Badell-Grau, Sophie R. Cook, Kimberley M. Jones, Emily D. Kirkham, Jincy Winston, Brendan P. Kelly   +8 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Adiabatic Flip-Flops and Sequential Circuit Design using Novel Resettable Adiabatic Buffers [PDF]

, 2017
We propose novel resettable adiabatic buffers for five adiabatic logic families namely; Efficient Adiabatic Charge Recovery Logic (EACRL), Improved Efficient Charge Recovery Logic (IECRL), Positive Feedback Adiabatic Logic (PFAL), Complementary Pass ...
Sachin Maheshwari, Kale, I., Izzet Kale, Maheshwari, S., Bartlett, V., V. A. Bartlett   +5 more
core   +1 more source

The Cyclical Behaviour of European Bank Capital Buffers [PDF]

Using an unbalanced panel of accounting data from 1997 to 2004 and controlling for individual bank costs and risk, we find capital buffers of the banks in the EU15 have a significant negative co-movement with the cycle.
Jokipii, Terhi, Milne, Alistair
core   +2 more sources

Arañas en cultivos de lechuga con cilantro como planta acompañante

AIA Avances en Investigación Agropecuaria
Objetivo. Este estudio busca relevar la composición, abundancia, gremios, variaciones estacionales y diversidad de arañas en cultivos de lechuga con cilantro como planta acompañante. Materiales y métodos.
Victoria Fernández Acevedo, Andrea Armendano   +1 more
doaj   +1 more source

Effects of Sodium Bicarbonate Supplementation on Physical Performance and Cardiovascular Variables in Middle-Aged and Elderly Adults with Type 2 Diabetes Mellitus [PDF]

Brazilian Archives of Biology and Technology
The global epidemic of diabetes mellitus (DM) presents a significant health challenge, and physical exercise is crucial in managing type 2 DM (T2DM). Sodium bicarbonate (SB) supplementation may enhance high-intensity exercise performance.
Edher Lucas Antunes, Rafael Carlos Sochodolak, Maria Luiza Pinto Rios, Jéssica Mendes Nadal, Paulo Vitor Farago, Nilo Massaru Okuno   +5 more
doaj   +2 more sources