Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Assessing the energy performance of HVAC systems in the tertiary building sector by on - site monitoring [PDF]
The paper discusses the collection and processing of energy performance data as part of the inspection of HVAC systems, aimed at identifying technically feasible and cost-effective Energy Conservation Opportunities (ECOs), as required by the European ...
ROSCETTI, ANDREA +27 more
core +2 more sources
Multiscale modeling of smectite illitization in bentonite buffer of engineered barrier system
With the increasing usage of nuclear energy, how to properly dispose nuclear waste becomes a critical issue. In this study, a multiscale modeling approach combining the experimental findings is presented to address the illitization process, its impact on
Xinwei Xiong +3 more
doaj +1 more source
Analysis of Environmental Aspects in High Energy Performance Family House - Case Study
In general, high energy performance building design ranges from architectural design to the application of technologies for energy conversation. Many applications are focused on insulation of facades, roofs and floors to those that are used systems for ...
S. Vilcekova +5 more
doaj +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Green remodeling projects for public buildings in South Korea aim to improve the energy performance of aging facilities, with a policy target of achieving at least 30% energy savings. Because the 30% saving rate functions as a key policy benchmark, it is
Jihoon Jang +3 more
doaj +1 more source
Assessment of energy utilization and leakages in buildings with building information model energy
Given the ability of building information models (BIM) to serve as a multidisciplinary data repository, this study attempts to explore and exploit the sustainability value of BIM in delivering buildings that require less energy for operations, emit less ...
Egwunatum I. Samuel +2 more
doaj +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Missed Opportunities in Building Energy Performance Assessment
Buildings continue to be important contributors to global greenhouse gas (GHG) emissions, despite the continued increase in stringency of energy and emissions codes and standards worldwide to address energy use in buildings.
Minu Agarwal +3 more
doaj +1 more source

