Results 51 to 60 of about 105,339 (264)

Laboratory and Reservoir-Scale Assessment of Thermal–Gas–Chemical Treatment Using Activated Aluminum Alloys at the Karazhanbas Field

Energies
Thermal–gas–chemical treatment (TGCT) using activated aluminum alloys is a promising near-wellbore stimulation method for high-viscosity oil reservoirs, combining localized heat generation, hydrogen release, pressure increase, and chemical activation of ...
Karlygash Soltanbekova, Galina Boiko, Raushan Sarmurzina, Nina Lyubchenko, Nariman Sarsenbekov, Askhat Khasenov   +5 more
doaj   +1 more source

TOWARD BUILDING KNOWLEDGE FOR SUPPORTING SUSTAINABLE TRANSITION OF THE BUILDING STOCK

SGEM International Multidisciplinary Scientific GeoConference EXPO Proceedings, 2014
The paper discusses the possibility of developing an easy and shareable framework with the aim of building basic knowledge for supporting the energy retrofitting process of the heritage building stock, with spatial detail at the neighborhood or district scale and, in a more general sense, for supporting the urban regeneration process.
openaire   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy

FEBS Open Bio, EarlyView.
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan, Ikram A. Burney, Shika Hanif Malgundkar, Siva Ramamoorthy, Ressin Varghese, Mohamed A. Al‐Kindi, Hamad Al Riyami, Shadia Al Bahlani, Sergey Dobretsov, Syed Imran Hassan, Benjamin K. Tsang, Yahya Tamimi   +11 more
wiley   +1 more source

Reducing Friction with a Liquid Film on the Body Surface

Lubricants, 2018
A flow of a thin layer of liquid is simulated on a flat surface of a body located in a stream of air. Liquid film on the surface of the body reduces frictional resistance and can be used as a boundary layer control element.
Nikolay Klyuev, Konstantin Polyakov, Alyona Krutovertseva   +2 more
doaj   +1 more source

Decarbonising the Welsh housing stock: from practice to policy

Buildings & Cities, 2020
This paper examines whether and how the challenging target of 95% decarbonisation can be achieved for the Welsh building stock. First, a critical review of the literature and relevant case studies are presented to understand the effectiveness of ...
Ed Green, Simon Lannon, Jo Patterson, Fabrizio Varriale, Heledd Iorwerth   +4 more
doaj   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Urban embodied carbon assessment: methodology and insights from analyzing over a million buildings in Chicago

Carbon Management
Confronting climate change, this study quantifies the embodied carbon emissions from the building sector—an overlooked yet significant contributor to urban carbon emissions.
Siavash Ghorbany, Ming Hu
doaj   +1 more source