Results 61 to 70 of about 1,334,119 (308)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Policies to decarbonize the Swiss residential building stock: An agent-based building stock modeling assessment

Energy Policy, 2020
Abstract In light of the Swiss government's reduction targets for greenhouse gas (GHG) emissions under the Paris Agreement, this article investigates how and with which policy measures these reduction targets can be met for the Swiss residential building sector.
Claudio Nägeli, Martin Jakob, Giacomo Catenazzi, York Ostermeyer   +3 more
openaire   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

EXPERIMENTAL DETERMINATION OF THE HYDROGEN CONCENTRATION IN THE BATTERY BOXES OF THE PASSENGER CARS

Nauka ta progres transportu, 2010
In the work the experimental determination of the hydrogen concentration in accumulator boxes of the coach in a charging mode of nickel-cadmium batteries in operating conditions (stop and operation) is presented.
G. S. Ighnatov, V. R. Raspopin, S. D. Sychev, S. V. Shmakov, P. A. Khozja, A. A. Sulim, A. A. Melnyk   +6 more
doaj   +2 more sources

Influence of street setbacks on solar reflection and air cooling by reflective streets in urban canyons [PDF]

, 2017
The ability of a climate model to accurately simulate the urban cooling effect of raising street albedo may be hampered by unrealistic representations of street geometry in the urban canyon.
Ban-Weiss, G, Levinson, RM, Mohegh, A, Rosado, PJ   +3 more
core   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Environmental evaluation of energy efficiency refurbishment in New Zealand's commercial office buildings : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy in Science in Life Cycle Management at Massey University, Manawatū, New Zealand [PDF]

, 2017
In New Zealand, 80 % of existing commercial office buildings are more than 20 years old and consume approximately 40 % more energy than newer counterparts.
Ghose, Agneta
core  

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Decarbonising the Welsh housing stock: from practice to policy

Buildings & Cities, 2020
This paper examines whether and how the challenging target of 95% decarbonisation can be achieved for the Welsh building stock. First, a critical review of the literature and relevant case studies are presented to understand the effectiveness of ...
Ed Green, Simon Lannon, Jo Patterson, Fabrizio Varriale, Heledd Iorwerth   +4 more
doaj   +1 more source