Sequence differences in the diagnostic target region of the oocyst wall protein gene of Cryptosporidium parasites [PDF]
Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from Various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine,
Sulaiman, I. +5 more
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Evaluation of electrolyzed water to control fungal trunk pathogens in grapevine nurseries
Electrolyzed water treatments could have promising applications in the grapevine nursery process to prevent or reduce infections caused by fungal trunk pathogens on grapevine propagation material. Abstract BACKGROUND Grapevine producers demand solutions to control fungal trunk pathogens (FTPs) in nurseries.
Mónica Berbegal +6 more
wiley +1 more source
Simplified methods for obtaining purified oocysts from mice and for growing Cryptosporidium parvum in vitro [PDF]
Seven- to 8-day-old Arc/Swiss mice were infected with 100,000-120,000 Cryptosporidium parvum oocysts. At 8 days postinfection (PI) the jejunum, ileum, cecum, colon, and rectum were removed.
Meloni, B.P., Thompson, R.C.A.
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Transgenic selection and underlying mechanisms in apicomplexan parasites
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju +2 more
wiley +1 more source
Cryptosporidium tyzzeri and Cryptosporidium pestis: Which name is valid? [PDF]
The dispute on the validity of Cryptosporidium pestis and Cryptosporidium tyzzeri origins from the uncertainty on the identity of Cryptosporidium parvum described by Tyzzer in 1912 and the interpretation of the Principal of Priority of the International ...
Fayer, R. +4 more
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PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin [PDF]
Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species.
SPANO F +4 more
openaire +4 more sources
Cross‐sectional study among 70 sexual health clinic attendees (70% MSM) in Bucharest combining multisite STI testing with knowledge, attitudes, and practices assessment. Key findings include 35.5% bacterial STI prevalence, essential extragenital testing, and cost barriers to screening uptake.
Andrei Tanasov, George‐Sorin Tiplica
wiley +1 more source
The apicomplexan Cryptosporidium parvum causes thousands of human deaths yearly. Since bovines represent the most important reservoir of C. parvum, the analysis of infected bovine small intestinal (BSI) explants cultured under physioxia offers a ...
Juan Vélez +6 more
doaj +1 more source
ABSTRACT Aim Risk assessment tools for periodontitis lack sufficient precision to predict disease progression. The aim of this exploratory study was to evaluate proteolytic activity (PA) in gingival crevicular fluid in relation to clinically assessed progression risk, microbial community composition and host proteome. Materials and Methods Samples were
Miguel Carda‐Diéguez +10 more
wiley +1 more source
Transient transfection of Cryptosporidium parvum using green fluorescent protein (GFP) as a marker [PDF]
Cryptosporidium parvum is a protozoan parasite that infects a variety of mammals. The parasite has been shown to harbor a dsRNA virus (CPV) and in the present study, we have developed a CPV transient transfection system for this parasite by using green ...
Yu, X. +8 more
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