Cold Adaptation of a Mesophilic Subtilisin-like Protease by Laboratory Evolution [PDF]
, 2000 Enzymes isolated from organisms native to cold environments generally exhibit higher catalytic efficiency at low temperatures and greater thermosensitivity than their mesophilic counterparts.
Arnold, Frances H. +2 more
core +1 more source
FEBS Open Bio, EarlyView.Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Sifat Fisik Daging Domba Yang Diberi Perlakuan Stimulasi Listrik Voltase Rendah Dan Injeksi Kalsium Klorida [PDF]
, 2004 Effect of low voltage electrical stimulation (LVES; 45 volt, 3 amps for approx 100 sec) and calcium chloride (CaCl2) injection on physical properties of meat were evaluated. Six mutton were devided into 3 groups.
Astawan, M. (M) +2 more
core +1 more source
Development of an Ion-Sensor using Fluorescence Resonance Energy Transfer
, 2014 A method is presented for the sensing of ions by determining the concentration of corresponding salts (KCl, NaCl, MgCl2, CaCl2, FeCl3, FeSO4, AlCl3) in water, based on Fluorescence resonance energy transfer (FRET) process.
Arpan Datta Roy +4 more
core +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Application and Ethephon of Calcium Chloride for Improving the Fruit Quality of Pineapple [PDF]
, 2013 The objective of research was to obtain the treatment time of CaCl2 applications, dosage of CaCl2 and ethephon applications to improve the fruit quality of pineapple. This research used Split-Split Plot Design and each treatment replicated 3times.
Astutl, N. K. (Nungki) +2 more
core
Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz +3 more
wiley +1 more source
Tenderness and flavor of leg cuts from meat goats influenced by calcium chloride injection
International Journal of Food Properties, 2018 This study was conducted to assess the potential of improving tenderness of chevon using calcium chloride (CaCl2) injection and its effect on the palatability characteristics of chevon.
J. H. Lee, G. Kannan, B. Kouakou
doaj +1 more source
Establishment of a cryopreservation process for long-term storage of embryogenic calli of Hevea brasiliensis (Müll. Arg.). [PDF]
, 2006 A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The technique included a callus pre-culture on a maintenance medium containing 1 mM CaCl2 prior to cryopreservation to promote postthaw callus
Carron, Marc-Philippe +4 more
core
Harnessing Fungal Biowelding for Constructing Mycelium‐Engineered Materials
Advanced Engineering Materials, EarlyView.Mycelium‐bound composites (MBCs) offer low‐carbon alternatives for construction, yet interfacial bonding remains a critical challenge. This review examines fungal biowelding as a biocompatible adhesive, elucidating mycelium‐mediated interfacial mechanisms and their role in material assembly. Strategies to optimize biowelding are discussed, highlighting
Xue Brenda Bai +2 more
wiley +1 more source