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Nature Methods, 2021
The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.
Nina Vogt
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The near-infrared calcium sensor iGECI shows promise for imaging neuronal activity in vivo.
Nina Vogt
semanticscholar +3 more sources
Calcium imaging with chemiluminescence
Microscopy Research and Technique, 1999This review updates the imaging of free cytosolic calcium with the chemiluminescent aequorins. Basic principles of chemiluminescence are discussed and the biochemistry of aequorins is briefly described. The review provides practical tips on handling and microinjecting aequorins and describes available ultra low light imaging systems.
R, Créton, J A, Kreiling, L F, Jaffe
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Calcium imaging of gut activity
Neurogastroenterology & Motility, 2004AbstractThe major cell types regulating gut motility include enteric neurones, interstitial cells of Cajal (ICC) and their effector smooth muscle cells. These cells are arranged conveniently in nested layers through the gut wall. Our knowledge of how many of these cells in each layer are integrated to produce the various patterns of motility is largely
J, Tack, T K, Smith
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Calcium Imaging in Mouse Superior Colliculus
Journal of Visualized Experiments, 2023The superior colliculus (SC), an evolutionarily conserved midbrain structure in all vertebrates, is the most sophisticated visual center before the emergence of the cerebral cortex. It receives direct inputs from ~30 types of retinal ganglion cells (RGCs), with each encoding a specific visual feature.
Zhe, Li, Ruixiang, Wu, Ya-Tang, Li
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Blood cells, 1993
We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
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We review herein the following questions related to Ca2+ imaging: what are the key elements to consider in a Ca2+ imaging system? What are the limits of such systems? What are the main artifacts and to what specific applications are a Ca2+ imaging systems best adapted?
A, Trautmann, Y P, Tan
openaire +1 more source
Calcium Imaging of Non-adherent Cells
2023Live-cell imaging can reveal dynamic and multimodal cell signaling by monitoring calcium flux. Spatiotemporal changes in Ca2+ concentrations instigate specific downstream processes and by categorizing these events, we can examine the language cells use to communicate both to themselves and with each other.
Bye, Lydia J. +2 more
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2003
As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
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As our understanding of the biological sciences expands, the boundaries between traditional disciplines tend to blur at the edges. Physiologists and pharmacologists, for instance, now need to embrace techniques that until recently were the strict preserves of biochemists and molecular biologists.
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Ex vivo calcium imaging in Drosophila opens an expansive amount of research avenues for the study of live signal propagation through complex tissue. Here, we describe how to isolate Drosophila organs of interest, like the developing wing imaginal disc and larval brain, culture them for extended periods, up to 10 h, and how to image the calcium dynamics
David V, Gazzo, Jeremiah J, Zartman
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David V, Gazzo, Jeremiah J, Zartman
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