Results 131 to 140 of about 160,656 (237)

Rapid and Efficient Affinity Isolation of Engineered Extracellular Vesicles Encapsulating Functional Proteins via Internal Twin‐Strep Tetraspanin Tagging

Small, EarlyView.
Structure‐guided internal twin‐Strep tagging of the tetraspanins CD63 or CD9 enables rapid and efficient antibody‐free affinity isolation of engineered extracellular vesicles (EVs) using Strep technology while allowing luminal protein loading. The approach removes over 96% of contaminants, separates tagged EVs from coexisting viral particles, and ...
Minh‐Tu Pham, Jona Benjamin Krohn, Janis Meyer, Christian Ritter, Martin Schneider, Dominic Helm, Dario Frey, Kleopatra Rapti, Uta Haselmann‐Weiss, Elif Toprak, Viet Loan Dao Thi, Florian Leuschner, Dirk Grimm, Karl Rohr, Ralf Bartenschlager   +14 more
wiley   +1 more source

Humidity Ramp Protocols Systematically Underestimate Human Heat Tolerance Limits

Safety Science and Technology, EarlyView.
Short dwell times in humidity‐ramp protocols (Δt/τ≪1 ${\Delta }t/\tau \mathit{\ll }1$) prevent human core temperature from reaching equilibrium, causing premature inflection points and systematic underestimation of critical environmental limits by ∼3°C–4°C.
Fèlix Faming Wang, Yi Xu, Haojian Wang, Min Cui, Xue Hou, Boan Wei, Xiong Shen   +6 more
wiley   +1 more source

Evaluating Thiram‐Induced Embryotoxicity Using Integrated In Silico, In Vitro, and Transcriptomic Approaches

Environmental Toxicology, EarlyView.
ABSTRACT Long‐term exposure to low‐dose food contact materials (FCMs) has raised concerns regarding developmental toxicity. In the present study, we prioritized FCMs with potential developmental toxicity using a weight‐of‐evidence computational model, which predicted 127 chemicals to be of high concern.
Chia‐Chi Ho, Chun‐Wei Tung, B. Linju Yen, Chen‐Yi Weng, Ju‐Hsin Hsu, Ming‐Hsien Tsai, Salim Arrokhman, Pinpin Lin   +7 more
wiley   +1 more source

Assessing veterinary students’ attitudes towards pain in cattle before and after final‐year clinical training

Veterinary Record, EarlyView.
Abstract Background Cattle only show subtle signs of pain, making pain assessment difficult. The approach to pain and the use of analgesics depend on the treating person's experience and opinions. Methods Bavarian veterinary students were asked to answer two identical questionnaires before and after 12 weeks of clinical training in a ruminant clinic. A
Theresa Tschoner, Kristina R. Mueller, Yury Zablotski, Melanie Feist   +3 more
wiley   +1 more source

FACTORS AFFECTING THE ADOPTION OF VALUE-ADDED PRODUCTION ON COW-CALF FARMS [PDF]

Factors that affect the decision to feed or sell calves at weaning are analyzed for Arkansas cow-calf operators. A discrete choice logit model is used to analyze the adoption of value-added cattle production.
Popp, Michael P., Parsch, Lucas D., Faminow, Merle D.   +2 more
core  

Safety of and serological response to a new canine distemper‐parvovirus combination vaccine in domestic ferrets

Veterinary Record, EarlyView.
Abstract Background Pet ferrets are typically vaccinated against distemper using a canine multivalent vaccine despite the lack of relevant safety or efficacy data. Here, we investigate the safety of a novel live canine parvovirus type 2c (CPV‐2c) vaccine strain and the efficacy of the live Onderstepoort canine distemper virus (CDV) vaccine strain used ...
Jacqueline Pearce, Léon Peeters, Ellen Versmissen, David Sutton, Ian Tarpey   +4 more
wiley   +1 more source

Temporal and seasonal trends of bovine tuberculosis in the Irish cattle population (2008–2024)

Veterinary Record, EarlyView.
Abstract Background Bovine tuberculosis (bTB) has substantial impacts on the Irish cattle industry. Between 2016 and 2024, its burden increased in Irish cattle. As an initial step towards understanding drivers of this increase, we explore long‐term trends in measures of bTB burden and cattle demographics in Ireland. Methods Using data from 2008 to 2024,
Jamie M. Madden, Eamonn Gormley, Philip Breslin, Damien Barrett, Jonas Brock, Jamie A. Tratalos, John Griffin, Mike Horan, Miriam Casey‐Bryars   +8 more
wiley   +1 more source

Suitable habitat of Himalayan wolf in Upper Mustang, Annapurna Conservation Area, Nepal

Wildlife Biology, EarlyView.
Decades ago, the Himalayan wolf Canis lupus chanco, a genetically distinct sub‐species of the gray wolf Canis lupus, faced persecution by local communities in the Nepalese Himalayas. Recently, wolf populations have returned and recolonized, sparking concerns about conflicts over livestock depredation, and emphasizing the urgent need for comprehensive ...
Deu Bahadur Rana, Jhamak Bahadur Karki, HimLal Shrestha, Thakur Silwal, Bikash Ghimire, Bikram Shrestha   +5 more
wiley   +1 more source

Behavioral response of moose Alces alces to summer heat stress

Wildlife Biology, EarlyView.
Minnesota moose are heat‐stressed during summers, which is adversely impacting their survival. We used both movement and activity data to explore the behavioral responses of Minnesota moose experiencing heat stress, termed ‘hot moose events' or HMEs, to understand how both ambient temperature and available habitat contribute to the ability of these ...
Michelle Carstensen, Amanda M. McGraw, Andrew N. Tri, Deahn M. Donner   +3 more
wiley   +1 more source

Sources of Eustress in Captivity

Zoo Biology, EarlyView.
Zoo environments foster enhanced animal welfare, integrated conservation, and improved population sustainability through the provision of eustressful factors (e.g., enrichment, social choice, positive human‐animal relationships), thus promoting cognitive challenge, improved resilience, and positive life experiences.
Paul Rose
wiley   +1 more source