We screened 558 reverse transcriptases and engineered an optimized rat endogenous retrovirus‐derived variant, enRERV‐RT, via structure‐guided engineering and deep mutational scanning. This enhanced prime editor, based on the engineered RT, outperforms conventional M‐MLV‐RT systems across plant and animal cells, particularly at hard‐to‐edit loci ...
Linsha Ma +22 more
wiley +1 more source
Is heterosis noticeable in the callus response of winter durum wheat F<sub>1</sub> hybrids? [PDF]
The effect of heterosis on callus induction, callus mass and number of regenerated plants from mature embryo cultures of winter durum wheat (Triticum durum Desf.) hybrids was studied. A total of 14 F1 hybrids and their parents were used for mature embryo
A. Ozbay, M. Özgen
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Induction and microscopic structure of embryogenic callus. [PDF]
A: Cones of immature larch; B: Inoculation of explants; C: Callus(EC: Embryogenic callus; Non-embryogenic callus, NEC), D: Microstructure of non-embryogenic callus; E: Initiation of EC-white mucilaginous tissue protruded from the micropylar end of the ...
Shanshan Yan (761415) +7 more
core +1 more source
PRODUCTION OF SALT-TOLERANT SOUR ORANGE PLANTS (Citrus aurantium L.) USING TISSUE CULTURE TECHNIQUE: II- IN VITRO EVALUATION AND SELECTION OF SALT TOLERANT CALLUS [PDF]
This study was conducted through 1996-1999 years in the Department of Pomology, Faculty of Agriculture, Alexandria University. The work involved the evaluation and selection of salt tolerant callus of sour orange under in vitro conditions.
N. Mustafa, M. El-Sabrout, A. El-Gazzar,
doaj +1 more source
A periosteum‐inspired Janus piezoelectric scaffold converts micromechanical forces into electrical signals and synergizes with controlled resveratrol release. This dual action reprograms endothelial metabolism, drives H‑type vessel formation, and restores bone–vessel coupling in diabetic bone defects.
Kai Wang, Kai Jiang
wiley +1 more source
Morphogenetic routes of long-term embryogenic callus culture of Areca catechu [PDF]
Early morphogenetic events and repetitive embryogenesis from callus culture of betel nut palm (Areca catechu L.) were studied using scanning electron microscopy.
J. T. Chen, H. C. Wang, W. C. Chang
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A Conserved DT2‐bZIP66‐NF‐YC4 Regulatory Module Confers Drought Tolerance in Rice and Arabidopsis
This study identifies a conserved tripartiteDT2–bZIP66–NF‐YC4 transcriptional module that enhances rice drought tolerance by activating stress‐responsive genes. This regulatory complex is functionally conserved across cereals and Arabidopsis, providing promising targets for engineering drought‐resilient crops. ABSTRACT Drought stress severely restricts
Jun Shen +18 more
wiley +1 more source
Soybean Callus—A Potential Source of Tocopherols [PDF]
In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were ...
Teodor Rusu +4 more
core +1 more source
Synergistic HMGN1 and VP64 Fusions Potentiate High‐Precision and PAM‐Flexible Base Editing
A novel CDA1Δ‐SpRY architecture fused with HMGN1 and VP64 yields a nearly PAM‐less base editing platform. By focusing cytosine conversion predominantly at position −18, this synergistic complex ensures highly precise targeting. Demonstrating enhanced efficiency across diverse models, including yeast and rice, the platform offers a robust solution for ...
Xi Luo +11 more
wiley +1 more source
Calos de passifloras silvestres: indução, caracterização morfo-histológica, metabólica e ultraestrutural [PDF]
Dissertação (mestrado) - Universidade Federal de Santa Catarina, Centro de Ciências Biológicas, Programa de Pós-Graduação em Biologia de Fungos, Algas e Plantas, Florianópolis, 2015.Passiflora setacea e P.
Wolfart, Márcia Regina
core

