Results 101 to 110 of about 3,906,604 (254)

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Exploring the influence of lactoferrin on wound healing and related signaling pathways: a systematic review

FEBS Open Bio, EarlyView.
Lactoferrin promotes wound healing by activating MAPK, PI3K/Akt, and extracellular matrix pathways, enhancing fibroblast remodeling, proliferation, and re‐epithelialization. These mechanisms highlight its therapeutic potential in inflammation control, tissue repair, and remodeling.
Morgana Lüdtke Azevedo, Kátia Cristiane Hall, Matheus dos Santos Fernandez, Geovanna Peter Corrêa, Rafael Guerra Lund   +4 more
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

CAP: Commutative algebra prediction of protein-nucleic acid binding affinities

Machine Learning: Science and Technology
An accurate prediction of protein-nucleic acid binding affinity is vital for deciphering genomic processes, yet existing approaches often struggle in reconciling high accuracy with interpretability and computational efficiency.
Mushal Zia, Faisal Suwayyid, Yuta Hozumi, JunJie Wee, Hongsong Feng, Guo-Wei Wei   +5 more
doaj   +1 more source

Expression of recombinant Cap antigen of porcine circovirus type 2 in the chloroplast of Chlamydomonas reinhardtii

Frontiers in Plant Science
The capsid (Cap) protein encoded by the ORF2 gene of porcine circovirus type 2 is the major immunogen for the development of vaccines and can effectively reduce the incidence of porcine circovirus-associated diseases.
Yiran Song, Youhong Lin, Xiaying Cheng, Wei Li, Yihong Li, Zongqi Yang   +5 more
doaj   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Determining biophysical protein stability in lysates by a fast proteolysis assay, FASTpp. [PDF]

, 2012
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp).
Rüdiger, Stefan G. D., Stefan G D Rüdiger, Maurice, Madelon M., David P Minde, Sub Cellular Protein Chemistry, Stefan G. D. Rüdiger, Madelon M Maurice, Minde, David P., Cellular Protein Chemistry, David P. Minde, Madelon M. Maurice   +10 more
core   +3 more sources

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

A novel mode of capping protein-regulation by twinfilin

eLife, 2018
Cellular actin assembly is controlled at the barbed ends of actin filaments, where capping protein (CP) limits polymerization. Twinfilin is a conserved in vivo binding partner of CP, yet the significance of this interaction has remained a mystery. Here, we discover that the C-terminal tail of Twinfilin harbors a CP-interacting (CPI) motif, identifying ...
Adam B Johnston, Denise M Hilton, Patrick McConnell, Britney Johnson, Meghan T Harris, Avital Simone, Gaya K Amarasinghe, John A Cooper, Bruce L Goode   +8 more
openaire   +4 more sources

The splicing factor proline-glutamine rich (SFPQ/PSF) is involved in influenza virus transcription. [PDF]

, 2011
The influenza A virus RNA polymerase is a heterotrimeric complex responsible for viral genome transcription and replication in the nucleus of infected cells. We recently carried out a proteomic analysis of purified polymerase expressed in human cells and
Juan Ortín, Landeras-Bueno, Sara, Jorba, N, Sara Landeras-Bueno, Ortín Juan, Pérez-Cidoncha, Maite, Pérez-Cidoncha, M, Núria Jorba, Landeras-Bueno Sara, Pérez-Cidoncha Maite, Landeras-Bueno, S, Ortín, J, Ortín, Juan, Jorba, Núria, Maite Pérez-Cidoncha, Jorba Núria   +15 more
core   +2 more sources