Results 71 to 80 of about 8,566,253 (298)
D-CAPS: an efficient CRISPR-Cas9-based phage defense system for E. coli
Acta Biochimica et Biophysica SinicaEscherichia coli is widely used in industrial chemical synthesis but faces significant challenges due to bacteriophage contamination, which reduces product quality and yield. Therefore, developing an efficient antiphage system is essential. In this study,
Sun Mingjun +7 more
doaj +1 more source
Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control
FEBS Open Bio, EarlyView.Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada +5 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
FEBS Open Bio, EarlyView.Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
THE SHEAR BEHAVIOUR OF THE REINFORCED CONCRETE FOUR-PILE CAPS
, 2009 There has been a consistent discrepancy between UK design standards BS5400 and BS8110 in the prediction of the shear capacity of 2-way spanning reinforced concrete pile caps from bending theory-based empirical design formulae.
Cao, Jing
core
FEBS Open Bio, EarlyView.This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Hedging with interest rate caps compared with a policy of maintaining a balanced portfolio of loans (PLA) and averaging the borrowing costs [PDF]
This paper compares two different strategies for managing interest rate exposure. One involves maintaining a borrowing portfolio using short and long term debt lines in order to maintain an average borrowing cost.
David E. Allen, Quinten Steyn
core
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
2015/2018 CAPS Assessment (Usage Reports and Data)
, 2019 CAPS Report 2015/2016, 2016/2017, 2017 ...
CAPS
core
, 2014 Left: PCC co-activation patterns in wakefulness (colormap normalized by BOLD intensity), current dataset (18 subjects). Note the similarity of these patterns with the ones showed in Fig. 2 of [16].
Carol Di Perri (307818) +9 more
core +1 more source