A Global Survey of Carbohydrate Esterase Families 1 and 10 in Oomycetes [PDF]
Frontiers in Genetics, 2020 Carbohydrate-active enzymes (CAZymes) are a cornerstone in the phytopathogenicity of filamentous microbes. CAZymes are required for every step of a successful infection cycle—from penetration, to nutrient acquisition (during colonization), to exit and ...
Sophie de Vries +3 more
doaj +7 more sources
Functional Validation of Two Fungal Subfamilies in Carbohydrate Esterase Family 1 by Biochemical Characterization of Esterases From Uncharacterized Branches [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The fungal members of Carbohydrate Esterase family 1 (CE1) from the CAZy database include both acetyl xylan esterases (AXEs) and feruloyl esterases (FAEs). AXEs and FAEs are essential auxiliary enzymes to unlock the full potential of feedstock.
Xinxin Li +8 more
doaj +12 more sources
Structural, Biochemical, and Phylogenetic Analysis of Bacterial and Fungal Carbohydrate Esterase Family 15 Glucuronoyl Esterases in the Rumen [PDF]
Protein JournalGlucuronoyl esterases (GEs) are carbohydrate active enzymes in carbohydrate esterase family 15 which are involved in the hydrolysis of lignin-carbohydrate complexes.
Gruninger RJ +6 more
exaly +5 more sources
Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification [PDF]
Frontiers in Plant Science, 2020 Fast-growing broad-leaf tree species can serve as feedstocks for production of bio-based chemicals and fuels through biochemical conversion of wood to monosaccharides. This conversion is hampered by the xylan acetylation pattern.
Zhao Wang +7 more
doaj +3 more sources
Structural Characterization and Directed Evolution of a Novel Acetyl Xylan Esterase Reveals Thermostability Determinants of the Carbohydrate Esterase 7 Family [PDF]
Applied and Environmental Microbiology, 2018 ABSTRACT A hot desert hypolith metagenomic DNA sequence data set was screened in silico for genes annotated as acetyl xylan esterases (AcXEs). One of the genes identified encoded an ∼36-kDa protein (Axe1 NaM1 ).
Fiyinfoluwa A Adesioye +2 more
exaly +7 more sources
The natural catalytic function of CuGE glucuronoyl esterase in hydrolysis of genuine lignin–carbohydrate complexes from birch [PDF]
Biotechnology for Biofuels, 2018 Background Glucuronoyl esterases belong to carbohydrate esterase family 15 and catalyze de-esterification. Their natural function is presumed to be cleavage of ester linkages in lignin–carbohydrate complexes particularly those linking lignin and ...
Caroline Mosbech +3 more
doaj +4 more sources
Structural and biochemical insights into the catalytic mechanisms of two insect chitin deacetylases of the carbohydrate esterase 4 family [PDF]
Journal of Biological Chemistry, 2019 Insect chitin deacetylases (CDAs) catalyze the removal of acetyl groups from chitin and modify this polymer during its synthesis and reorganization.
Ming-Bo Qu, Yuebin Zhang, Tian Liu
exaly +3 more sources
Biochemical Characterization of a Family 15 Carbohydrate Esterase from a Bacterial Marine Arctic Metagenome. [PDF]
PLoS ONE, 2016 The glucuronoyl esterase enzymes of wood-degrading fungi (Carbohydrate Esterase family 15; CE15) form part of the hemicellulolytic and cellulolytic enzyme systems that break down plant biomass, and have possible applications in biotechnology.
Concetta De Santi +2 more
doaj +3 more sources
Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds [PDF]
Microbial Cell FactoriesBackground The conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial saccharification and cellular metabolism. Trichoderma spp.
Yang Liu +6 more
doaj +3 more sources
Structural and functional investigation of a fungal member of carbohydrate esterase family 15 with potential specificity for rare xylans. [PDF]
Acta Crystallogr D Struct Biol, 2023 Structural investigation of a presumed fungal glucuronoyl esterase reveals a classical serine hydrolase active site but an unusual ligand-binding site. Functional analysis showed a lack of activity on a wide array of substrates commonly utilized by this ...
Mazurkewich S +8 more
europepmc +2 more sources