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Carbohydrate-active enzymes: sequences, shapes, contortions and cells

Biochemical Society Transactions, 2016
The enzyme-catalysed degradation of oligo and polysaccharides is of considerable interest in many fields ranging from the fundamental–understanding the intrinsic chemical beauty–through to the applied, including diverse practical applications in medicine and biotechnology.
Spencer J. Williams, Gideon J. Davies
openaire   +4 more sources

Use of mass spectrometry for the carbohydrate composition and sequence analysis of glycosphingolipids

Biological Mass Spectrometry, 1974
Mass spectrometry has been applied for a detailed structural characterization of glycosphingolipids. Methylated and methylated plus reduced glycolipids (amide groups of ceramide and amino sugars were reduced to the corresponding amines) were found the most useful derivatives.
I. Pascher   +3 more
openaire   +4 more sources

The monosaccharide composition and sequence of the carbohydrate moiety of human serum low density lipoproteins

Biochemistry, 1976
Human serum low density lipoprotein (d = 1.027-1.045) was delipidated with organic solvents and the apoprotein digested with thermolysin. The digest was fractionated by gel filtration and DEAE-cellulose chromatography. Two glycopeptides were obtained. One of the glycopeptides (GP-I) contained 2 residues of N-acetylglucosamine and 6 residues of mannose ...
N. Swaminathan, Frederick Aladjem
openaire   +4 more sources

Mass spectrometry for structural elucidation and sequencing of carbohydrates

TrAC Trends in Analytical Chemistry, 2021
Abstract Carbohydrates are the most abundant organic compounds in nature, widely distributed in animals, plants and microorganisms. In order to understanding their structure properties, the following critical aspects should be revealed: a) building blocks (compositional monosaccharide); b) glycosidic linkages and anomeric configuration; c) sequence ...
Shao-Ping Li   +5 more
openaire   +2 more sources

Low-carbohydrate, ketogenic diet impairs anaerobic exercise performance in exercise-trained women and men: a randomized-sequence crossover trial.

Journal of Sports Medicine and Physical Fitness, 2019
BACKGROUND Low-carbohydrate, ketogenic diets cause mild, subclinical systemic acidosis. Anaerobic exercise performance is limited by acidosis. Therefore, we evaluated the hypothesis that a low-carbohydrate, ketogenic diet impairs anaerobic exercise ...
K. Wroble   +5 more
semanticscholar   +1 more source

GlycoCT—a unifying sequence format for carbohydrates

Carbohydrate Research, 2008
As part of the EUROCarbDB project (www.eurocarbdb.org) we have carefully analyzed the encoding capabilities of all existing carbohydrate sequence formats and the content of publically available structure databases. We have found that none of the existing structural encoding schemata are capable of coping with the full complexity to be expected for ...
Stephan Herget   +3 more
openaire   +3 more sources

Fluorophore-assisted carbohydrate electrophoresis in the separation, analysis, and sequencing of carbohydrates

Journal of Chromatography A, 1996
Carbohydrate analysis has traditionally been viewed as a specialty science, performed only in a few well-established laboratories using conventional carbohydrate analysis technology (e.g. NMR, gas chromatography-mass spectroscopy, high-performance liquid chromatography, capillary electrophoresis) combined with the specialized technical training that ...
Christopher M. Starr   +4 more
openaire   +3 more sources

Sequence-Based Prediction of Protein-Carbohydrate Binding Sites Using Support Vector Machines.

Journal of Chemical Information and Modeling, 2016
Carbohydrate-binding proteins play significant roles in many diseases including cancer. Here, we established a machine-learning-based method (called sequence-based prediction of residue-level interaction sites of carbohydrates, SPRINT-CBH) to predict ...
G. Taherzadeh   +3 more
semanticscholar   +1 more source

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