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[16] Carbohydrate sequence analysis by electrospray ionization-mass spectrometry
1996Abstract This chapter summarizes several strategies for a more complete understanding of carbohydrate structure with a focus on N-linked glycans. The techniques include periodate oxidation to impart greater molecular specificity, functional group blocking by methylation, electrospray for “soft” and efficient ionization, collision-induced dissociation
Stephen Chan +2 more
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Human Urinary Kallikrein: Amino Acid Sequence and Carbohydrate Attachment Sites
1989The amino acid sequence of human urinary kallikrein was determined by protein-chemical methods. The peptide chain contains 238 amino acid residues. Ten half-cystine residues form five disulfide bridges. Six carbohydrate side chains could be identified, three of them are N-glycosidically linked to asparagine which is present in the acceptor sequon Asn ...
J Kellermann +3 more
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The Journal of Biochemistry, 1994
The primary sequence of 247 amino acids of Maackia amurensis hemagglutinin (MAH) was determined using a protein sequencer. After digestion with endoproteinase Lys-C, Asp-N, Arg-C, or Glu-C of MAH, the resulting peptides were purified by reversed phase high performance liquid chromatography (HPLC) and then subjected to sequence analysis.
Tatsuro Irimura +4 more
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The primary sequence of 247 amino acids of Maackia amurensis hemagglutinin (MAH) was determined using a protein sequencer. After digestion with endoproteinase Lys-C, Asp-N, Arg-C, or Glu-C of MAH, the resulting peptides were purified by reversed phase high performance liquid chromatography (HPLC) and then subjected to sequence analysis.
Tatsuro Irimura +4 more
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Handling and Conversion of Carbohydrate Sequence Formats and Monosaccharide Notation
2015Various glycobioinformatics resources have developed individual carbohydrate sequence formats to store and handle glycan data. This diversity of sequence formats is one of the major reasons for a rather low interoperability of glycobioinformatics resources.
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Glycoprofiling by DNA Sequencer-Aided Fluorophore-Assisted Carbohydrate Electrophoresis
2007In recent years, the importance of glycosylation has been realized. Carbohydrates not only serve as decoration for proteins and lipids but also form an integral part of the glycoconjugate, adding to its functional and structural properties. The specific type of glycosylation may change with altering physiological conditions, such as disease.
Roland Contreras, Wouter Laroy
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European Journal of Organic Chemistry, 2018
Carbohydrates serve as versatile molecular scaffolds and platforms in organic chemistry, for example in combinatorial chemistry or for the multivalent presentation of ligands. Carbohydrate scaffolds are frequently employed in the synthesis of multivalent glycoconjugates and utilized for the study of fundamental parameters of carbohydrate recognition ...
Anna K. Ciuk +2 more
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Carbohydrates serve as versatile molecular scaffolds and platforms in organic chemistry, for example in combinatorial chemistry or for the multivalent presentation of ligands. Carbohydrate scaffolds are frequently employed in the synthesis of multivalent glycoconjugates and utilized for the study of fundamental parameters of carbohydrate recognition ...
Anna K. Ciuk +2 more
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Carbohydrate-active enzymes in completely sequenced genomes [PDF]
Biochemical Society Transactions, 2002 Bernard Henrissat, Bernard Henrissat
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Cancer epigenetics in clinical practice
Ca-A Cancer Journal for Clinicians, 2023Veronica Davalos, Manel Esteller
exaly
Hierarchical Nanomaterials Assembled from Peptoids and Other Sequence-Defined Synthetic Polymers
Chemical Reviews, 2021Zhiliang Li, Bin Cai, Wenchao Yang
exaly