Results 71 to 80 of about 771,008 (307)

Core and auxiliary functions of one-carbon metabolism in Pseudomonas putida exposed by a systems-level analysis of transcriptional and physiological responses

mSystems, 2023
The soil bacterium Pseudomonas putida is a robust biomanufacturing host that assimilates a broad range of substrates while efficiently coping with adverse environmental conditions. P. putida is equipped with functions related to one-carbon (C1) compounds
Justine Turlin, Òscar Puiggené, Stefano Donati, Nicolas T. Wirth, Pablo I. Nikel   +4 more
doaj   +1 more source

Starve Cancer Cells of Glutamine: Break the Spell or Make a Hungry Monster? [PDF]

, 2019
Distinct from normal differentiated tissues, cancer cells reprogram nutrient uptake and utilization to accommodate their elevated demands for biosynthesis and energy production.
Jiang, Jie, Srivastava, Sankalp, Zhang, Ji   +2 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus. [PDF]

, 2020
Filamentous fungi, such as Neurospora crassa, are very efficient in deconstructing plant biomass by the secretion of an arsenal of plant cell wall-degrading enzymes, by remodeling metabolism to accommodate production of secreted enzymes, and by enabling ...
Benz, J Philipp, Blow, Matthew J, Calhoun, Sara, Dietschmann, Axel, Glass, N Louise, Grigoriev, Igor V, Huberman, Lori B, Kowbel, David J, Lee, Juna, Lipzen, Anna, Monti, Remo, O'Malley, Ronan C, Singan, Vasanth R, Thieme, Nils, Wu, Vincent W, Xiong, Yi   +15 more
core   +3 more sources

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

The compositional and evolutionary logic of metabolism

, 2012
Metabolism displays striking and robust regularities in the forms of modularity and hierarchy, whose composition may be compactly described. This renders metabolic architecture comprehensible as a system, and suggests the order in which layers of that ...
Alberts B, Andreini C, Balche W E, Barker H A, Benner S A, Berge C, Braakman R, Brazelton W J, Buss L W, Collins M D, Copley S D, Dagley S, Darwin C, de Duve C, Doyle J, Eric Smith, Erwin D H, Feist A M, Fenchel T, Fisher R A, Fry I, Gerhart J, Gesteland R F, Gould S J, Gray H B, Guiral M, Haldane J B S, Kallen R G, Kauffman S, Kim J, Lengeler J W, Ljungdahl L, MacKenzie R E, Martin W, Massey L K, Metzler D E, Morowitz H J, Morowitz H J, Morowitz H J, Nelson D L, Nitscke W, Odling-Smee F J, Oparin A I, Petsko G A, Puigbo P, Ragsdale S, Ragsdale S W, Rankama K, Razin S, Redfield A C, Redfield A C, Rogier Braakman, Russell M J, Schrödinger E, Schuster P, Segré D, Shapiro R, Simon H A, Simon H A, Smith E, Srinivasan V, Srinivasan V, Sterner R W, Stryer L, Szent-Gyorgyi A, Tazuya K, Utter M F, Vogels G D, von Wettstein D, Vorholt J A, Waber L J, Wald G, Wilson E B, Wächtershäuser G, Yarus M, Yi L   +75 more
core   +1 more source

Resistance to BRAF inhibitors induces glutamine dependency in melanoma cells [PDF]

, 2015
BRAF inhibitors can extend progression-free and overall survival in melanoma patients whose tumors harbor mutations in BRAF. However, the majority of patients eventually develop resistance to these drugs. Here we show that BRAF mutant melanoma cells that
Baenke, Franziska, Chaneton, Barbara, Dhomen, Nathalie, Galbraith, Laura, Girotti, Maria R., Gottlieb, Eyal, Hogan, Kate, Marais, Richard, Martin, Matthew, Smith, Matthew, Tang, Haoran, Van Den Broek, Niels, Viros, Amaya   +12 more
core   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source

Straw and biochar strongly affect functional diversity of microbial metabolism in paddy soils

Journal of Integrative Agriculture, 2019
The application of straw and biochar is widely practiced for the improvement of soil fertility. However, its impact on microbial functional profiles, particularly with regard to paddy soils, is not well understood.
Hong-zhao YUAN, Zhen-ke ZHU, Xiao-meng WEI, Shou-long LIU, Pei-qin PENG, Anna Gunina, Jian-lin SHEN, Yakov Kuzyakov, Ti-da GE, Jin-shui WU, Jiu-rong WANG   +10 more
doaj   +1 more source

Medium alkalization due to carbon metabolism is largely responsible for inhibition of bacterial growth by Vibrio cholerae supernatants [PDF]

, 2017
Vibrio cholerae is the causative agent of the diarrheal disease cholera. Many Vibrio species secrete antimicrobial factors, though the identity of such a factor has not been determined for any V. cholerae strain.
Becker, Miranda
core   +1 more source