Results 101 to 110 of about 537 (137)
Molecular analysis of the interaction between cardosin A and phospholipase Dα [PDF]
FEBS Journal, 2005 Cardosin A is an RGD-containing aspartic proteinase from the stigmatic papillae of Cynara cardunculus L. A putative cardosin A-binding protein has previously been isolated from pollen suggesting its potential involvement in pollen2013pistil interaction [Faro C, Ramalho-Santos M, Vieira M, Mendes A, Simões I, Andrade R, Verissimo P, Lin X, Tang J ...
Carlos Faro +2 more
exaly +6 more sources
Functional and structural characterization of synthetic cardosin B-derived rennet
Applied Microbiology and Biotechnology, 2017 The potential of using a synthetic cardosin-based rennet in cheese manufacturing was recently demonstrated with the development and optimization of production of a recombinant form of cardosin B in Kluyveromyces lactis. With the goal of providing a more detailed characterization of this rennet, we herein evaluate the impact of the plant-specific insert
Ana C Figueiredo +2 more
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Journal of Molecular Catalysis B: Enzymatic, 2009 Cardosin A (EC: 3.4.23) is an enzyme containing two polypeptide chains, purified from pistils of Cynara cardunculus L., a cardoon, used for milk clotting in cheese making. It is a member of the aspartic proteinases (APs), like pepsin and HIV-proteinase that are composed by two symmetric units comprising the active site.
Isabel Correia +2 more
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Hydrolysis of α-lactalbumin by cardosin A immobilized on highly activated supports [PDF]
Enzyme and Microbial Technology, 2003 In the present research effort, production of derivatives of cardosin A (a plant protease) encompassing full stabilization of its dimeric structure has been achieved, via covalent, multi-subunit immobilization onto highly activated agarose-glutaraldehyde supports.
Rui Barros +2 more
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Functional and conformational changes in the aspartic protease cardosin A induced by TFE
International Journal of Biological Macromolecules, 2012 Conformational and functional changes of cardosin A, an aspartic protease of vegetal origin, in the presence of 2,2,2-trifluoroethanol (TFE), were assessed. TFE induced alterations of cardosin activity and conformation that differed with the solvent concentration. MD simulations showed that there are significant local alterations in protein flexibility
Rui M. Brito, Euclides M.V. Pires
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On the activity and specificity of cardosin B, a plant proteinase, on ovine caseins [PDF]
Food Chemistry, 1999 The proteolytic activity of cardosin B, an aspartic proteinase from the thistle, Cynara cardunculus, on ovine α(s)-caseins and β-caseins (independently or present together in sodium caseinate) was followed by urea polyacrylamide gel electrophoresis and reversed phase high performance liquid chromatography.
Francisco Xavier Malcata
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Molecular cloning and characterization of cDNA encoding cardosin B, an aspartic proteinase accumulating extracellularly in the transmitting tissue of Cynara cardunculus L. [PDF]
Plant Molecular Biology, 2001 Cardosins A and B are related aspartic proteinases from the pistils of Cynara cardunculus L., whose milk-clotting activity has been exploited for the manufacture of cheese.
Pedro Castanheira +2 more
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Some of the next articles are maybe not open access.
Reverse hydrolysis by cardosin A: specificity considerations
Journal of Molecular Catalysis B: Enzymatic, 2004Cardosin A, a plant aspartic proteinase, capable of synthesising peptides, was investigated through synthesis of five methyl esters amino acid substrates as amino donors and nine benzyloxycarbonyl amino acid and peptide carboxyl donors. It was found that cardosin A is able to catalyse the synthesis of several peptide bonds, being the preference order ...
Francisco Amado +2 more
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The heterologous systems in the study of cardosin B trafficking pathways [PDF]
Plant Signaling and Behavior, 2011 Cardosins are abundant in cardoon pistils and were found to accumulate in different cell compartments: cardosin A was detected in the vacuoles of stigmatic papillae and cardosin B accumulates in the extracellular matrix of the transmitting tissue. Due to the fact that cardoon system imposes some limitations to the study of processing and trafficking ...
Diana Soares da Costa +2 more
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Dissecting cardosin B trafficking pathways in heterologous systems
Planta, 2010In cardoon pistils, while cardosin A is detected in the vacuoles of stigmatic papillae, cardosin B accumulates in the extracellular matrix of the transmitting tissue. Given cardosins' high homology and yet different cellular localisation, cardosins represent a potentially useful model to understand and study the structural and functional plasticity of ...
Ian Moore +2 more
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