Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Decolonization vs Enhanced Cleaning for Multidrug-Resistant Organism Reduction in Nursing Homes.
Gussin GM +11 more
europepmc +1 more source
Genomic diversity and topical antimicrobial resistance in <i>Staphylococcus aureus</i> from clinical and carriage populations in the New York-New Jersey region. [PDF]
Terlecky AJ +17 more
europepmc +1 more source
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata +13 more
wiley +1 more source
Serological evidence of high exposure to <i>Plasmodium falciparum</i> gameto-cytes in dry-Season in Kenieroba, Mali. [PDF]
Zeguime A +24 more
europepmc +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Prevalence, Clonal Context, and Clinical Correlates of Arr in <i>Klebsiella Pneumoniae</i> from a Specialized Infectious Disease Hospital. [PDF]
He L +7 more
europepmc +1 more source
Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar +4 more
wiley +1 more source
APOE-ε4 moderates the association between diet quality and executive function in middle-aged women at increased risk for Alzheimer's disease. [PDF]
Wessinger CM +8 more
europepmc +1 more source

