Pilot-scale Operation of a Multi-enzymatic Cascade Reaction in a Multiphase System
Chemical Engineering Transactions, 2020 This work aims to emphasize the importance of scale-up in biotechnological research, in order to introduce more environmentally friendly bio-catalyzed processes to the industry.
Jens Johannsen +5 more
doaj +4 more sources
Optimization of a reduced enzymatic reaction cascade for the production of L-alanine [PDF]
Scientific Reports, 2019 AbstractCell-free enzymatic reaction cascades combine the advantages of well-established in vitro biocatalysis with the power of multi-step in vivo pathways. The absence of a regulatory cell environment enables direct process control including methods for facile bottleneck identification and process optimization.
Tobias J. Gmelch +2 more
core +7 more sources
A Multi-Enzymatic Cascade Reaction for the Synthesis of Vidarabine 5′-Monophosphate [PDF]
Catalysts, 2020 We here described a three-step multi-enzymatic reaction for the one-pot synthesis of vidarabine 5′-monophosphate (araA-MP), an antiviral drug, using arabinosyluracil (araU), adenine (Ade), and adenosine triphosphate (ATP) as precursors. To this aim, three enzymes involved in the biosynthesis of nucleosides and nucleotides were used in a cascade mode ...
Marina Simona Robescu +4 more
core +4 more sources
A Cascade Enzymatic Reaction Scheme for Irreversible Transpeptidative Protein Ligation
Journal of the American Chemical Society, 2023 Enzymatic peptide ligation holds great promise in the study of protein functions and development of protein therapeutics. Owing to their high catalytic efficiency and a minimal tripeptide recognition motif, peptidyl asparaginyl ligases (PALs) are particularly useful tools for bioconjugation.
Yiyin Xia +8 more
openaire +5 more sources
A multi-enzymatic cascade reaction for the stereoselective production of γ-oxyfunctionalyzed amino acids [PDF]
Frontiers in Microbiology, 2016 A stereoselective three-enzyme cascade for synthesis of diasteromerically pure γ-oxyfunctionalized α-amino acids was developed. By coupling a dynamic kinetic resolution using an N-acylamino acid racemase and an L-selective aminoacylase from Geobacillus ...
Junichi eEnoki +3 more
doaj +3 more sources
DNA Adjuvant Hydrogel‐Optimized Enzymatic Cascade Reaction for Tumor Chemodynamic‐Immunotherapy
Advanced ScienceChemodynamic therapy (CDT) shows immense potential in cancer treatment as it not only directly kills tumor cells but also induces anti‐tumor immune responses.
Yan Zhao +5 more
doaj +3 more sources
A Bi-Enzymatic Cascade Pathway Towards FAHFAs [PDF]
, 2021 Recently discovered endogenous mammalian lipids fatty acid esters of hydroxy fatty acids (FAHFAs), proved to have anti-inflammatory and anti-diabetic effects. Due to their extremely low abundancies in vivo, forging a feasible scenario for FAHFA synthesis
Bekir Engin, Eser +2 more
core +2 more sources
Hydrogel Microvalves as Control Elements for Parallelized Enzymatic Cascade Reactions in Microfluidics [PDF]
Micromachines, 2020 Compartmentalized microfluidic devices with immobilized catalysts are a valuable tool for overcoming the incompatibility challenge in (bio) catalytic cascade reactions and high-throughput screening of multiple reaction parameters. To achieve flow control in microfluidics, stimuli-responsive hydrogel microvalves were previously introduced.
Franziska Obst +6 more
openaire +3 more sources
Nanoscale Enzymatic Compartments in Tandem Support Cascade Reactions in Vitro [PDF]
Biomacromolecules, 2018 Compartmentalization at the nanoscale is fundamental in nature, where the spatial segregation of biochemical reactions within cells ensures optimal conditions for regulating metabolic pathways. Here, we present a nature-inspired approach to engineer enzymatic cascade reactions taking place between separate vesicular nanocompartments (polymersomes ...
Andrea Belluati +3 more
openaire +4 more sources
Membraneless Compartmentalization Facilitates Enzymatic Cascade Reactions and Reduces Substrate Inhibition [PDF]
ACS Applied Materials & Interfaces, 2018 Living cells possess membraneless organelles formed by liquid-liquid phase separation. With the aim of better understanding the general functions of membraneless microcompartments, this paper constructs acellular multicompartment reaction systems using an aqueous multiphase system.
Taisuke Kojima, Shuichi Takayama
openaire +2 more sources