Reconstructing enzyme evolution by protein engineering
FEBS Letters, EarlyView.Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler +2 more
wiley +1 more source
Case Study on the Impact of Technology on Incivility in Higher Education
Journal of Educators Online, 2016 A qualitative case study research design provided an in-depth perspective of the participants in relation to understanding the holistic impact technology has on the incivility of student-tostudent and student-to-faculty interactions in higher education ...
Michelle Bartlett, James E. Bartlett
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Applications of Operations Research to A Check Processing System
Operations Research ...
Svestka, Joseph A. (Creatorcre)
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Investigating transcription factor dynamics in health and disease using FRAP
FEBS Letters, EarlyView.FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj +3 more
wiley +1 more source
Case study research for better evaluations of complex interventions: rationale and challenges. [PDF]
BMC Med, 2020 Paparini S +7 more
europepmc +1 more source
Allocation of Resources for Basic Research in a Mission Oriented Governmental Agency
Operations Research ...
Brown, Melvin (Creatorcre) +4 more
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Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential
FEBS Letters, EarlyView.Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta +3 more
wiley +1 more source
An Application of the Matrix Method in Physiological Psychology Research Planning
Operations Research ...
Mathis, Samuel J., Jr. (Creatorcre) +3 more
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Structure‐forward targeting of claudins with synthetic binders
FEBS Letters, EarlyView.Claudins form the paracellular barriers between epithelial and endothelial tissues at tight junctions and are targets for molecular binders with the goal of modulating barrier permeability. Claudin‐binding molecules are relevant in drug delivery or in altering claudin interactions with disease‐causing proteins.
Alex J. Vecchio
wiley +1 more source
Interview with Arch Woodside on Case study research: theory, methods and practice, by Arch Woodside
As discussed in this interview, Case study research: theory, methods and practice looks at the research processes involved in conducting methods including: participant observation; fuzzy set social science; decision systems analysis; forced metaphor ...
Woodside, Arch
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