Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Disordered but rhythmic—the role of intrinsic protein disorder in eukaryotic circadian timing
Unstructured domains known as intrinsically disordered regions (IDRs) are present in nearly every part of the eukaryotic core circadian oscillator. IDRs enable many diverse inter‐ and intramolecular interactions that support clock function. IDR conformations are highly tunable by post‐translational modifications and environmental conditions, which ...
Emery T. Usher, Jacqueline F. Pelham
wiley +1 more source
Detailed flow measurements in casing boundary layer of 429-meter-per-second-tip-speed two-stage fan [PDF]
Detailed flow measurements between all blade rows were taken in the outer 30 percent of passage height of a two stage fan. Tabulations of the detailed flow measurements are included.
Gorrell, W. T.
core +1 more source
Field Measurements of Spontaneous Potential (SP) for Smart Well Monitoring and Control. A Field Test in the UK Chalk Aquifer [PDF]
Imperial Users ...
Esquivel Garcia, Armando +1 more
core
To develop a new mineral carbonation process that have a high efficiency in CO2 absorption into industry slag using low energy mechanical milling [PDF]
Increase in the CO2 emission in atmosphere due to the combustion of fossil fuels has caused serious global warming. Electricity generation, tranportation, and industrial waste are the main sectors indentified to contribute to the emission of CO2 in ...
Selamat, Siti Norhana
core
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
The present study was conducted from September, 2019 to April, 2020 at College of Horticulture and Forestry, Neri, Hamirpur, Himachal Pradesh, India with an aim to examine the chemical parameters of various casing substrates and their changes throughout
Manisha Saini +2 more
doaj +1 more source
Study of Turbofan Engines Designed for Low Enery Consumption [PDF]
Subsonic transport turbofan engine design and technology features which have promise of improving aircraft energy consumption are described. Task I addressed the selection and evaluation of features for the CF6 family of engines in current aircraft, and ...
Hirschkron, R. +2 more
core +1 more source
Telemedicine as a special case of Machine Translation
Machine translation is evolving quite rapidly in terms of quality. Nowadays, we have several machine translation systems available in the web, which provide reasonable translations.
Glinkowski, Wojciech +2 more
core +1 more source
Multidrug transporters BpeB and BpeF from the Gram‐negative pathogen Burkholderia pseudomallei have a hydrophilic patch in their substrate‐binding pocket. Drug susceptibility tests and growth curve analyses using an Escherichia coli recombinant expression system revealed that the hydrophilic patches of BpeB and BpeF are involved in the substrate ...
Ui Okada, Satoshi Murakami
wiley +1 more source

