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Imaging MS Analysis in Catharanthus roseus

2022
To understand how the plant regulates metabolism, it is important to determine where metabolites localize in the tissues and cells. Single-cell level omics approaches in plants have shown remarkable development over the last several years, and this data has been instrumental in gene discovery efforts for enzymes and transporters involved in metabolism.
Yamamoto, K.   +3 more
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Production of anthocyanins by Catharanthus roseus

Fitoterapia, 2003
A stable cell suspension line of Catharanthus roseus producing anthocyanin was obtained. In this strain it was found that approximately 30% of cells regularly accumulated these metabolites and that anthocyanin accumulation occurred between the second half of log phase and the stationary phase of the culture growth cycle.
FILIPPINI, RAFFAELLA   +3 more
openaire   +5 more sources

Codon usage bias of Catharanthus roseus

China Journal of Chinese Materia Medica, 2016
This study aimed to provide guidance for the heterogenous gene expression, gene prediction and species evolution by analyzing codon usage bias of Catharanthus roseus.The codon composition and usage bias of 30 437 high-confidence coding sequences from C.roseus were analyzed and the proportion of rare codons of Escherichia coli and Saccharomyces ...
Ying, Li   +4 more
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Glyphosate-tolerance in Catharanthus roseus

Plant Science, 1988
Abstract Cultured cells of Catharanthus roseus were selected by a stepwise procedure for tolerance to the herbicide glyphosate. The selected cells were found to contain levels of extractable 5-enolpyruvylshikimic acid-3-phosphate (EPSP) synthase activity significantly greater than those found in non-selected cells.
R.C. Cresswell, M.W. Fowler, A.H. Scragg
openaire   +1 more source

Studying Iridoid Transport in Catharanthus roseus by Grafting

2022
The plant Catharanthus roseus is well known for its spatial separation of iridoid and monoterpenoid indole alkaloid (MIA) biosynthesis at both intracellular and intercellular levels, collectively suggested by RNA in situ hybridization, enzymatic and transcriptomic studies using leaf epidermis, and fluorescent protein tagging studies.
Maisha, Farzana   +3 more
openaire   +2 more sources

Alkaloid metabolism in wounded Catharanthus roseus seedlings

Plant Physiology and Biochemistry, 2004
The effect of mechanical wounding on alkaloid metabolism was analyzed in Catharanthus roseus seedlings. Wounding induced an increase in ajmalicine accumulation, whereas catharanthine remained unaffected. A positive dual effect on vindoline was noticed.
Felipe, Vázquez-Flota   +3 more
openaire   +2 more sources

Proteome analysis of the medicinal plant Catharanthus roseus

Planta, 2005
A proteomic approach is undertaken aiming at the identification of novel proteins involved in the alkaloid biosynthesis of Catharanthus roseus. The C. roseus cell suspension culture A11 accumulates the terpenoid indole alkaloids strictosidine, ajmalicine and vindolinine.
Denise I, Jacobs   +4 more
openaire   +2 more sources

The proton-pumps at the plasmalemma of Catharanthus roseus cells

Biochimie, 1986
Cultured Catharanthus roseus cells exhibit transmembrane ferricyanide (FIC) reduction which is associated with a proton translocation and a decrease in the ATP content of the cells. The H+ efflux and the ATP consumption may be counteracted by vanadate, a specific inhibitor of the ATPase activity, and by Na2WO4 which prevents FIC reduction.
M, Belkoura, G, Marigo
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Biosynthesis of brassinosteroids in cultured cells of Catharanthus roseus

Phytochemistry, 2000
Precursor administration experiments with 2H-labeled 6-oxocampestanol, 6-deoxocastasterone and 6alpha-hydroxycastasterone in cultured cells of Catharanthus roseus were performed and the metabolites were analyzed by GC-MS. [2H6]Cathasterone was identified as a metabolite of [2H6]6-oxocampestanol, whereas [2H6]6alpha-hydroxycastasterone and [2H6 ...
S, Fujioka   +4 more
openaire   +2 more sources

Catharanthus roseus

2023
Ramachandra Reddy Pamuru   +5 more
openaire   +2 more sources

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