Results 11 to 20 of about 86,415 (162)

Understanding domain swapping in the c-Src SH3 domain through hinge-loop mutagenesis. [PDF]

Acta Crystallogr D Struct Biol
The role of the hinge loop and the open interface in the 3D domain swapping of the c‐Src SH3 domain is reported.The c‐Src SH3 domain is one of the best‐characterized modular domains from a biophysical and structural point of view. This SH3 domain displays noncanonical alternative folding, forming 3D domain‐swapped oligomers and amyloid fibrils.
Salinas-Garcia MC, Plaza-Garrido M, Martinez JC, Camara-Artigas A.   +3 more
europepmc   +2 more sources

NOTCH3 Mutation Causes Glymphatic Impairment and Promotes Brain Senescence in CADASIL. [PDF]

CNS Neurosci Ther
Beside mural cells, NOTCH3 is expressed in astrocytes. Expression of Aquaporin 4 (AQP4), the core mediator of glymphatic functions, is decreased in CADASIL brain. Consequently, glymphatic activities are impaired. Diminished AQP4 expression is attributed to the suppression of RUNX1‐CMYB‐AQP4 signaling due to NOTCH3 mutation in astrocytes.
Li C, Li H, Men X, Wang Y, Kang X, Hu M, Su X, Wang S, Lu D, Shen S, Huang H, Deng X, Liu Y, Zhang L, Cai W, Wu A, Lu Z.   +16 more
europepmc   +2 more sources

Crystal structures of 40- and 71-substitution variants of hydroxynitrile lyase from rubber tree. [PDF]

Acta Crystallogr D Struct Biol
Variants HNL16, HNL40 and HNL71 of hydroxynitrile lyase from Hevea brasiliensis contain 16, 40 and 71 mutations, respectively, to make increasingly larger regions surrounding the active site identical in sequence to the esterase SABP2. X‐ray structures of HNL40 and HNL71 reveal changes to the oxyanion hole and a new tunnel, which may contribute to ...
Pierce CT, Tan P, Greenberg LR, Walsh ME, Shi K, Nguyen AH, Meixner EL, Sarak S, Aihara H, Evans RL, Kazlauskas RJ.   +10 more
europepmc   +2 more sources

Fast event-based electron counting for small-molecule structure determination by MicroED. [PDF]

Acta Crystallogr C Struct Chem
Fast readout event‐based electron counting (EBEC) is a promising detection strategy to determine accurate ab initio structures of beam‐sensitive small molecules by MicroED. A fast EBEC approach enhances the dynamic range of MicroED data by limiting the likelihood of coincidence loss (CL) – the undercounting of electrons due to their spatially and ...
Vlahakis N, Qu S, Richards LS, Moraes LS, Cascio D, Nelson HM, Rodriguez JA.   +6 more
europepmc   +2 more sources

The crystal structure of Shethna protein II (FeSII) from Azotobacter vinelandii suggests a domain swap. [PDF]

Acta Crystallogr D Struct Biol
The Azotobacter vinelandii FeSII protein forms an oxygen‐resistant complex with the nitrogenase MoFe and Fe proteins.The Azotobacter vinelandii FeSII protein forms an oxygen‐resistant complex with the nitrogenase MoFe and Fe proteins. FeSII is an adrenodoxin‐type ferredoxin that forms a dimer in solution.
Kabasakal BV, McFarlane CR, Cotton CAR, Schmidt A, Kung A, Lieber L, Murray JW.   +6 more
europepmc   +2 more sources

Setting of an import tolerance for lambda‐cyhalothrin in avocados

EFSA Journal, Volume 21, Issue 12, December 2023., 2023
Abstract In accordance with Article 6 of Regulation (EC) No 396/2005, the applicant Syngenta Crop Protection AG submitted a request to the competent national authority in Greece to set an import tolerance for the active substance lambda‐cyhalothrin in avocados.
EFSA European Food Safety Authority (EFSA), Giulia Bellisai, Giovanni Bernasconi, Luis Carrasco Cabrera, Irene Castellan, Monica del Aguila, Lucien Ferreira, German Giner Santonja, Luna Greco, Samira Jarrah, Renata Leuschner, Ileana Miron, Stefanie Nave, Ragnor Pedersen, Hermine Reich, Silvia Ruocco, Miguel Santos, Alessia Pia Scarlato, Marta Szot, Anne Theobald, Manuela Tiramani, Alessia Verani   +21 more
wiley   +1 more source

Peer review of the pesticide risk assessment of the active substance bixlozone. [PDF]

EFSA J
Abstract The conclusions of the European Food Safety Authority (EFSA) following the peer review of the initial risk assessments carried out by the competent authority of the rapporteur Member State, The Netherlands for the pesticide active substance bixlozone are reported. The context of the peer review was that required by Regulation (EC) No 1107/2009
European Food Safety Authority (EFSA), Álvarez F, Arena M, Auteri D, Leite SB, Binaglia M, Castoldi AF, Chiusolo A, Colagiorgi A, Colas M, Crivellente F, De Lentdecker C, De Magistris I, Egsmose M, Fait G, Ferilli F, Santonja GG, Gouliarmou V, Halling K, Nogareda LH, Ippolito A, Istace F, Jarrah S, Kardassi D, Kienzler A, Lanzoni A, Lava R, Leuschner R, Linguadoca A, Louisse J, Lythgo C, Magrans O, Mangas I, Mioč A, Miron I, Molnar T, Padovani L, Padricello V, Panzarea M, Parra Morte JM, Rizzuto S, Romac A, Rortais A, Santos M, Serafimova R, Sharp R, Szentes C, Terron A, Theobald A, Tiramani M, Vianello G, Villamar-Bouza L.   +51 more
europepmc   +2 more sources

Atypical homodimerization revealed by the structure of the (S)‐enantioselective haloalkane dehalogenase DmmarA from Mycobacterium marinum

Acta Crystallographica Section D, Volume 79, Issue 11, Page 956-970, November 2023., 2023
Crystallographic structures of the (S)‐enantioselective haloalkane dehalogenase DmmarA from the waterborne pathogenic microbe Mycobacterium marinum were determined at 1.6 and 1.85 Å resolution. The structures reveal a previously unobserved mode of homodimerization, which is predominantly mediated through unusual L5‐to‐L5 loop interactions.Haloalkane ...
Karolina Snajdarova, Sérgio M. Marques, Jiri Damborsky, David Bednar, Martin Marek   +4 more
wiley   +1 more source

Increasing the bulk of the 1TEL–target linker and retaining the 10×His tag in a 1TEL–CMG2‐vWa construct improves crystal order and diffraction limits

Acta Crystallographica Section D, Volume 79, Issue 10, Page 925-943, October 2023., 2023
Using a 1TEL–CMG2‐vWa construct, evidence is provided to support limiting the flexibility of linkers between TELSAM and proteins of interest and considering retaining polyhistidine purification tags in TELSAM‐fusion constructs. The phenomenon of TELSAM‐polymer flipping is also identified and a correction strategy is developed.TELSAM‐fusion ...
Parag L. Gajjar, Maria J. Pedroza Romo, Celeste M. Litchfield, Miles Callahan, Nathan Redd, Supeshala Nawarathnage, Sara Soleimani, Jacob Averett, Elijah Wilson, Andrew Lewis, Cameron Stewart, Yi-Jie Tseng, Tzanko Doukov, Andrey Lebedev, James D. Moody   +14 more
wiley   +1 more source

Were there any tells in the Coţofeni culture area?

Българско е-Списание за Археология, 2022
The approach to the topic of tells in the North-Danube area, between 3500 and 2800 BC, corresponding to the Coţofeni culture (in Bulgaria, Magura–Coţofeni, Tărnava culture) has been inconsistent. This study aims to clarify a series of archaeological and
Cristian Ioan Popa
doaj   +1 more source