Results 71 to 80 of about 420,567 (309)
Development of human monoclonal antibodies against TARM1 by yeast display
FEBS Open Bio, EarlyView.Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Subtraction of cap-trapped full-length cDNA libraries to select rare transcripts
BioTechniques, 2003 The normalization and subtraction of highly expressed cDNAs from relatively large tissues before cloning dramatically enhanced the gene discovery by sequencing for the mouse fulllength cDNA encyclopedia, but these methods have not been suitable for ...
Tomoko Hirozane-Kishikawa +9 more
doaj +1 more source
Scientific Reports, 2021 RNA sequencing enables high-content/high-complexity measurements in small molecule screens. Whereas the costs of DNA sequencing and RNA-seq library preparation have decreased consistently, RNA extraction remains a significant bottleneck to scalability ...
Shreya Ghimire +3 more
doaj +1 more source
Generation and analysis of an Eucalyptus globulus cDNA library constructed from seedlings subjected to low temperature conditions [PDF]
, 2008 Indexación: ScieloEucalyptus globulus is the most important commercial temperate hardwood in the world because of its wood properties and due to its characteristics for biofuel production. However, only a very low number of expressed sequence tags (ESTs)
Gamboa, Maria Cecilia +4 more
core +2 more sources
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
3′ RACE Walking along a Large cDNA Employing Tiered Suppression PCR
BioTechniques, 2003 Large genes present particular cloning difficulties, especially when expressed at relatively low levels. We describe a novel method, termed 3′ rapid amplification of cDNA ends (RACE) walking, for the rapid determination of unknown 3′ flanking sequence of
Daniel J. Park +3 more
doaj +1 more source
Cloning and expression of porcine β1,4 N-acetylgalactosaminyl transferase encoding a new xenoreactive antigen [PDF]
, 2014 Xenograft rejection of pigs organs with an engineered mutation in the GGTA-1 gene (GTKO) remains a predominantly antibody mediated process which is directed to a variety of non-Gal protein and carbohydrate antigens.
Byrne, GW +4 more
core +2 more sources
FEBS Open Bio, EarlyView.Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu +10 more
wiley +1 more source
Preparation and quality detection on gene chip of head cDNA from Apis cerana cerana
浙江大学学报. 农业与生命科学版, 2009 Through sequencing of the head cDNA library of Chinese honeybee (Apis cerana cerana) and sequence analysis, 8569 high quality EST sequences were obtained.
YANG Yu-xiao +4 more
doaj +1 more source
RNomics: a computational search for box C/D snoRNA genes in the D. melanogaster genome. [PDF]
, 2004 Motivation: In eukaryotes, the family of non-coding RNA genes includes a number of genes encoding small nucleolar RNAs (mainly C/D and H/ACA snoRNAs), which act as guides in the maturation or post-transcriptional modifications of target RNA molecules ...
ACCARDO MC +6 more
core