Results 121 to 130 of about 4,481,323 (329)

A coiled‐coil domain triggers oligomerization of MmpL10, the mycobacterial transporter of trehalose polyphleate precursor

open access: yesFEBS Letters, EarlyView.
Most MmpLs transport lipids and glycolipids of the complex mycomembrane of mycobacteria. This study shows that MmpL10 from Mycobacterium smegmatis, the transporter of the trehalose polyphosphate precursor, harbors a coiled‐coil‐like extension. Biochemical and electron microscopy studies demonstrate that the coiled‐coil enables MmpL10 to trimerize.
Julie Couston   +7 more
wiley   +1 more source

A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons

open access: yesSTAR Protocols, 2020
Summary: Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders ...
Alessia Di Nardo   +2 more
doaj  

A simple, quantitative assay for the detection of viable but non-culturable (VBNC) bacteria

open access: yesSTAR Protocols, 2021
Summary: This protocol determines the fraction of a bacterial population that is viable and culturable, viable and non-culturable, or non-viable (dead).
Ignacio Servando Arvizu   +1 more
doaj  

An Optimized Immunohistochemistry Technique Improves NMO-IgG Detection: Study Comparison with Cell-Based Assays

open access: yesPLoS ONE, 2013
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays.
R. Höftberger   +10 more
semanticscholar   +1 more source

Flow‐based immunomagnetic enrichment of circulating tumor cells from diagnostic leukapheresis product

open access: yesMolecular Oncology, EarlyView.
The number of circulating tumor cells obtained from prostate cancer patients was increased approximately 5‐fold compared to regular CellSearch when processing 2 mL diagnostic leukapheresis material aliquots and increased by 44‐fold when processing 20 mL DLA aliquots using the flow enrichment target capture Halbach‐array.
Michiel Stevens   +8 more
wiley   +1 more source

Protocol for optimizing production and quality control of infective EcoHIV virions

open access: yesSTAR Protocols, 2023
Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production.
Hammodah R. Alfar   +12 more
doaj  

Cell-based assays for the detection of MOG antibodies: a comparative study

open access: yesJournal of Neurology, 2020
M. Gastaldi   +22 more
semanticscholar   +1 more source

High-Throughput 3-D Cellular Assays Using Destabilized Green Fluorescence Protein [PDF]

open access: yes, 2008
Cell assays for high-throughput screening (HTS) of potential drug candidates are important tools in the process of drug discovery. Most cellular assays are currently based on 2-D monolayer cell cultures, but 3-D cell cultures could better mimic the in ...
Fraley, Brian
core  

Unlocking the potential of tumor‐derived DNA in urine for cancer detection: methodological challenges and opportunities

open access: yesMolecular Oncology, EarlyView.
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever   +1 more
wiley   +1 more source

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