Results 121 to 130 of about 4,735,099 (379)

Cancer biomarkers, and novel techniques for detection [PDF]

, 2017
Technologies for early detection of tumors is critical for better therapy outcome and overall change in cancer survival. These assays must be capable of detecting tumors at early stages in order to prevent metastasis of the tumor and help reduce ...
Jamal, Tameem
core  

Alternative Methods for the Detection of Emerging Marine Toxins: Biosensors, Biochemical Assays and Cell-Based Assays

Marine Drugs, 2014
The emergence of marine toxins in water and seafood may have a considerable impact on public health. Although the tendency in Europe is to consolidate, when possible, official reference methods based on instrumental analysis, the development of ...
Laia Reverté, Lucía Soliño, Olga Carnicer, J. Diogène, M. Campàs   +4 more
semanticscholar   +1 more source

The anti‐CRISPR protein AcrIE8.1 inhibits the type I‐E CRISPR‐Cas system by directly binding to the Cascade subunit Cas11

FEBS Letters, EarlyView.
In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley   +1 more source

Targeted metabolomics in human and animal biofluids and tissues using liquid chromatography coupled with tandem mass spectrometry

STAR Protocols
Summary: Here, we present a targeted polar metabolomics protocol for the analysis of biofluids and frozen tissue biopsies using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS).
Jill A. Willency, Yanzhu Lin, Valentina Pirro   +2 more
doaj   +1 more source

Dual-acting stapled peptides target both HIV-1 entry and assembly [PDF]

, 2013
Background: Previously, we reported the conversion of the 12-mer linear and cell-impermeable peptide CAI to a cell-penetrating peptide NYAD-1 by using an i,i + 4 hydrocarbon stapling technique and confirmed its binding to the C-terminal domain (CTD ...
Bhargava, P., Cooper, A., Curreli, F., Debnath, A.K., Freed, E.O., Lee, S., Mehta, M., Mercredi, P.Y., Scacalossi, D., Summers, M.F., Tong, X., Waheed, A.A., Zhang, H.   +12 more
core   +2 more sources

Cell-based assays for Parkinson's disease using differentiated human LUHMES cells

Acta Pharmacologica Sinica, 2014
Aim:Lund human mesencephalic (LUHMES) cells can be differentiated to post-mitotic cells with biochemical, morphological and functional features of dopaminergic (DAergic) neurons.
Xiao-min Zhang, M. Yin, Min-hua Zhang
semanticscholar   +1 more source

By dawn or dusk—how circadian timing rewrites bacterial infection outcomes

FEBS Letters, EarlyView.
The circadian clock shapes immune function, yet its influence on infection outcomes is only beginning to be understood. This review highlights how circadian timing alters host responses to the bacterial pathogens Salmonella enterica, Listeria monocytogenes, and Streptococcus pneumoniae revealing that the effectiveness of immune defense depends not only
Devons Mo, Catherine S. Palmer, Jacqueline M. Kimmey   +2 more
wiley   +1 more source

Protocol to assess bioenergetics and mitochondrial fuel usage in murine autoreactive immunocytes using the Seahorse Extracellular Flux Analyzer

STAR Protocols
Summary: Efficient metabolism, or the means by which cells produce energy resources, is critical for proper effector function. Here, we present a protocol for examining the bioenergetics and mitochondrial fuel utilization of primary murine autoreactive ...
Colleen L. Mayberry, John J. Wilson, Britney Sison, Chih-Hao Chang   +3 more
doaj   +1 more source

Hematopoietic (stem) cells—The elixir of life?

FEBS Letters, EarlyView.
The aging of HSCs (hematopoietic stem cells) and the blood system leads to the decline of other organs. Rejuvenating aged HSCs improves the function of the blood system, slowing the aging of the heart, kidney, brain, and liver, and the occurrence of age‐related diseases.
Emilie L. Cerezo, Jonah Anderson, Emilie Dinh Vedrenne, Noël Yeh Martín, Jette Lengefeld   +4 more
wiley   +1 more source

Protocol to calculate the synergy of drug combinations in organoids and primary cells from murine tumors

STAR Protocols
Summary: Evaluating the synergy of drug combinations is crucial in advancing treatment regimens. Here, we present a protocol to establish primary cells and organoids from murine tumors and calculate drug synergy.
David Eisenbarth, Bomin Ku, Dae-Sik Lim
doaj   +1 more source