Results 121 to 130 of about 4,481,323 (329)
Most MmpLs transport lipids and glycolipids of the complex mycomembrane of mycobacteria. This study shows that MmpL10 from Mycobacterium smegmatis, the transporter of the trehalose polyphosphate precursor, harbors a coiled‐coil‐like extension. Biochemical and electron microscopy studies demonstrate that the coiled‐coil enables MmpL10 to trimerize.
Julie Couston+7 more
wiley +1 more source
A Cell-Based Assay Optimized for High-Content Cilia Imaging with Primary Rat Hippocampal Neurons
Summary: Genetic manipulations of dissociated rodent neurons provide translatable in vitro models for disease-driven phenotypes. Cilia are cellular antenna with a role in neuronal maturation and function often perturbed in neurodevelopmental disorders ...
Alessia Di Nardo+2 more
doaj
A simple, quantitative assay for the detection of viable but non-culturable (VBNC) bacteria
Summary: This protocol determines the fraction of a bacterial population that is viable and culturable, viable and non-culturable, or non-viable (dead).
Ignacio Servando Arvizu+1 more
doaj
Evidence for the presence of a receptor for the cytolethal distending toxin (CLDT) of Campylobacter jejuni on CHO and HeLa cell membranes and development of a receptor-based enzyme-linked immunosorbent assay for detection of CLDT [PDF]
P Bag
openalex +1 more source
Cell-based assays (CBA) have increased the sensitivity of the neuromyelitis optica (NMO)-IgG/aquaporin-4-antibody detection compared to classical tissue-based indirect assays.
R. Höftberger+10 more
semanticscholar +1 more source
The number of circulating tumor cells obtained from prostate cancer patients was increased approximately 5‐fold compared to regular CellSearch when processing 2 mL diagnostic leukapheresis material aliquots and increased by 44‐fold when processing 20 mL DLA aliquots using the flow enrichment target capture Halbach‐array.
Michiel Stevens+8 more
wiley +1 more source
Protocol for optimizing production and quality control of infective EcoHIV virions
Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production.
Hammodah R. Alfar+12 more
doaj
Cell-based assays for the detection of MOG antibodies: a comparative study
M. Gastaldi+22 more
semanticscholar +1 more source
High-Throughput 3-D Cellular Assays Using Destabilized Green Fluorescence Protein [PDF]
Cell assays for high-throughput screening (HTS) of potential drug candidates are important tools in the process of drug discovery. Most cellular assays are currently based on 2-D monolayer cell cultures, but 3-D cell cultures could better mimic the in ...
Fraley, Brian
core
Urine is a rich source of biomarkers for cancer detection. Tumor‐derived material is released into the bloodstream and transported to the urine. Urine can easily be collected from individuals, allowing non‐invasive cancer detection. This review discusses the rationale behind urine‐based cancer detection and its potential for cancer diagnostics ...
Birgit M. M. Wever+1 more
wiley +1 more source