Results 131 to 140 of about 27,881,538 (305)

Cell-based resorption assays for bone graft substitutes.

, 2012
The clinical utilization of resorbable bone substitutes has been growing rapidly during the last decade, creating a rising demand for new resorbable biomaterials. An ideal resorbable bone substitute should not only function as a load-bearing material but
Zhang, Z;Egana, JT;Reckhenrich, AK;Schenck, TL;Lohmeyer, JA;Schantz, JT;Machens, HG;Schilling, AF
core   +1 more source

Protocol for measuring mitochondrial respiratory capacity from buccal cell swabs

STAR Protocols
Summary: Mitochondrial respirometry provides a detailed assessment of oxygen consumption within the electron transport system, yet methods detailing respiration from non-invasive samples remain limited.
Tina R. Ram, Chunlong Mu, Jaclyn C. Campbell, Sarah J. MacEachern, Jane Shearer   +4 more
doaj   +1 more source

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Cell adhesion assays.

, 2009
This chapter will outline in detail the two standard assays used in the author's laboratory for quantitating the adhesion of cells to an immobilized substrate. The attachment assay, which employs a colorimetric detection of bound cells, is based on Kueng
Martin J. Humphries, Humphries, Martin J.   +1 more
core   +1 more source

Protocol for imaging-based quantification of RNAPII clearance during transcription-coupled DNA repair

STAR Protocols
Summary: Elongating RNA polymerase II (RNAPII) stalls at transcription-blocking lesions in the DNA template strand and is removed by transcription-coupled DNA repair (TCR) factors.
Bram A.F.J. de Groot, Paula J. van der Meer, Diana van den Heuvel, Martijn S. Luijsterburg   +3 more
doaj   +1 more source

Fixed cell-based assays for autoantibody detection in myasthenia gravis: a diagnostic breakthrough. [PDF]

Lancet Reg Health West Pac, 2023
Budhram A.
europepmc   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Mouse N2a Neuroblastoma Assay: Uncertainties and Comparison with Alternative Cell-Based Assays for Ciguatoxin Detection. [PDF]

Mar Drugs, 2023
Raposo-Garcia S, Cao A, Costas C, Louzao MC, Vilariño N, Vale C, Botana LM.   +6 more
europepmc   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

A high-throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell-based assays. [PDF]

Bioeng Transl Med, 2023
Kasper SH, Otten S, Squadroni B, Orr-Terry C, Kuang Y, Mussallem L, Ge L, Yan L, Kannan S, Verma CS, Brown CJ, Johannes CW, Lane DP, Chandramohan A, Partridge AW, Roberts LR, Josien H, Therien AG, Hett EC, Howell BJ, Peier A, Ai X, Cassaday J.   +22 more
europepmc   +1 more source