Results 131 to 140 of about 1,590,893 (316)

Micropatterned Porous Membranes for Combinatorial Cell-Based Assays

, 2014
International audienceHere, we describe a protocol for producing micropatterned porous membranes which can be used for combinatorial cell-based assays.
Di Meglio, Jean-Marc, Hersen, Pascal, Fanny Evenou, Pascal Hersen, Vulin, Clément, Clément Vulin, Jean Marc Di Meglio, Evenou, Fanny   +7 more
core   +1 more source

Protocol to study the direct binding of proteins to RNA:DNA hybrids or RNA-DNA chimeras in living cells using cross-linking immunoprecipitation

STAR Protocols
Summary: RNA-binding proteins (RBPs) are involved in many biological processes. The direct interaction between protein and RNA can be studied using cross-linking immunoprecipitation (CLIP) techniques in living cells.
Clara Bonnet, Ana Luisa Dian, Mélissa Leriche, Patricia Uguen, Stéphan Vagner   +4 more
doaj   +1 more source

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

A Cell-Based Assay to Measure the Activity of the Complement Convertases

Kidney International Reports
The complement system serves as a crucial defense mechanism against invading pathogens; however, dysregulation of this system can result in harmful consequences. Central to the complement cascade are the classical pathway (CP) or lectin pathway (LP) and the alternative pathway (AP) convertases. Aberrant regulation of the convertases is often implicated
Małgorzata Stasiłojć, Grzegorz Stasiłojć, Alicja Kuźniewska, Santiago Rodriguez de Córdoba, Marcin Okrój   +4 more
openaire   +4 more sources

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

An ultra scale-down tool set for the predictive design of a membrane separation procedure for preparation of human cell therapies

, 2015
Tools that allow cost-effective screening of the susceptibility of cell lines to operating conditions which may apply during full-scale processing are central to the rapid development of robust processes for cell-based therapies.
Masri, MF
core  

mRNA Transfection Retrofits Cell-Based Assays with Xenobiotic Metabolism

, 2018
Data associated with publication mRNA Transfection Retrofits Cell-Based Assays with Xenobiotic Metabolism.
EPA's National Center for Computational Toxicology (5039467)
core   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Processing of nanolitre liquid plugs for microfluidic cell-based assays

, 2012
Plugs, i.e. droplets formed in a microchannel, may revolutionize microfluidic cell-based assays. This study describes a microdevice that handles nanolitre-scale liquid plugs for the preparation of various culture setups and subsequent cellular assays. An
Junji Fukuda, Shintaro Takahashi, Tatsuya Osaki, Naoto Mochizuki and Hiroaki Suzuki
core   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source