Results 121 to 130 of about 1,374,920 (267)

Degradation mechanism of the von Willebrand factor A2 domain by nattokinase

FEBS Letters, EarlyView.
Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto, Masatomo So, Ayako Furukawa, Kenji Sugase   +3 more
wiley   +1 more source

Cutting edge strategies for screening of novel anti-HIV drug candidates against HIV infection: A concise overview of cell based assays. [PDF]

Heliyon, 2023
Gaikwad SY, Phatak P, Mukherjee A.
europepmc   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Elucidating EGCG' s targets in nasopharyngeal carcinoma through combined cell-based assays and transcriptomic sequencing. [PDF]

Discov Oncol
Yang Y, Xuan G, Feng Z, Deng M, Liu F, Liu Q, Wang W, Li Z, Zuo D, Li Z, Wei C, Luo W, Li F, Ma N, He F, Murata M.   +15 more
europepmc   +1 more source

Prozone phenomenon in pretransplant testing: An interesting conundrum involving solid-phase and cell-based assays. [PDF]

Asian J Transfus Sci, 2022
Singh P, Tiwari AK, Mishra VC, Deshpande TV, Dorwal P, Bhardwaj AK, Kumari S, Raina V.   +7 more
europepmc   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Consistent Detection of Aquaporin-4 Antibodies: A Comparative Analysis Between Fixed and Live Cell-Based Assays. [PDF]

J Clin Neurol
Wang J, Wang L, Yang X, Li Z, Jiang J, Xu Q, Huang S, Fu Q, Yang Y, Zhang R, Yang L, Chen A, Zeng X, Xu K, Zheng P, Qin X, Feng J.   +16 more
europepmc   +1 more source

Grouping of UVCB substances with dose-response transcriptomics data from human cell-based assays. [PDF]

ALTEX, 2022
House JS, Grimm FA, Klaren WD, Dalzell A, Kuchi S, Zhang SD, Lenz K, Boogaard PJ, Ketelslegers HB, Gant TW, Rusyn I, Wright FA.   +11 more
europepmc   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Extracellular Small RNAs in Human Milk: Molecular Profiles, Stability and Fragment-Specific Responses in Cell-Based Assays. [PDF]

Noncoding RNA
Claus C, Borini Etichetti C, Costa B, Grosso JB, Tosar JP, Chorostecki U, Spinelli SV.   +6 more
europepmc   +1 more source