Results 161 to 170 of about 1,959,228 (334)

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Protocol for identifying OXCT1-mediated LACTB succinylation sites in vitro

STAR Protocols
Summary: OXCT1 acts as a succinyltransferase to promote serine beta-lactamase-like protein (LACTB) K284 succinylation. Here, we present a protocol for detecting OXCT1-mediated LACTB succinylation levels and sites.
Wenhao Ma, Yuchen Sun, Huafeng Zhang
doaj   +1 more source

Cell-Based Assays to Identify ERR and ERR/PGC Modulators. [PDF]

Methods Mol Biol, 2022
Lynch C, Zhao J, Xia M.
europepmc   +1 more source

Detection of autoantibodies against the acetylcholine receptor, evaluation of commercially available methodologies: fixed Cell-Based Assay, Radioimmunoprecipitation Assay and Enzyme-Linked Immunosorbent Assay [PDF]

, 2023
Larissa Diogenes, Alessandra Dellavance, Danielle Cristiane Baldo, Sarah Cristina Gozzi-Silva, Kethellen Gomes, Mônica Simon Prado, Luís Eduardo Coelho Andrade, Gerson Dierley Keppeke   +7 more
openalex   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Antigen and Cell-Based Assays for the Detection of Non-HLA Antibodies. [PDF]

Front Immunol, 2022
Lammerts RGM, Altulea D, Hepkema BG, Sanders JS, van den Born J, Berger SP.   +5 more
europepmc   +1 more source

Electro-magnetic Templates with Magnetic Nanoparticles for Cell-based Assays

, 2016
Frederick Gertz, Alexander Khitun
openalex   +1 more source

β‐TrCP overexpression enhances cisplatin sensitivity by depleting BRCA1

Molecular Oncology, EarlyView.
Low levels of β‐TrCP (Panel A) allow the accumulation of BRCA1 and CtIP, which facilitate the repair of cisplatin‐induced DNA damage via homologous recombination (HR) and promote tumor cell survival. In contrast, high β‐TrCP expression (Panel B) leads to BRCA1 and CtIP degradation, impairing HR repair, resulting in persistent DNA damage and apoptosis ...
Rocío Jiménez‐Guerrero, Alejandro Belmonte‐Fernández, Mónica González‐Moreno, Laura Rodríguez‐Cordero, Begoña Pérez‐Valderrama, Joaquín Herrero‐Ruíz, Francisco Romero, Carmen Sáez, Miguel Á. Japón   +8 more
wiley   +1 more source

Pharmacologically active herbal remedies against atherosclerosis, characterization and DoE based marker quantification by densitometry, and cell based assays on THP-1 cell lines. [PDF]

Saudi Pharm J, 2023
Sireesha R, Krishnan M, Sadiq M, Ramanujam Ganesh M, Kamaraj C, Alharbi NS, Thiruvengadam M.   +6 more
europepmc   +1 more source

Discovery of Inhibitors of Microglial Neurotoxicity Acting Through Multiple Mechanisms Using a Stem-Cell-Based Phenotypic Assay [PDF]

, 2012
Susanne Höing, York Rudhard, Peter Reinhardt, Michael Glatza, Martin Stehling, Guangming Wu, Christiane Peiker, Alexander Böcker, Juan A. Parga, Eva C. Bunk, Jens C. Schwamborn, Mark Slack, Jared Sterneckert, Hans R. Schöler   +13 more
openalex   +1 more source