Results 161 to 170 of about 4,735,099 (379)

Evaluation of NK Cell Function by Flowcytometric Measurement and Impedance Based Assay Using Real-Time Cell Electronic Sensing System [PDF]

, 2013
Ki-Hyun Park, Hyesun Park, Myungshin Kim, Yonggoo Kim, Kyungja Han, Eun‐Jee Oh   +5 more
openalex   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Fixed cell-based assays for autoantibody detection in myasthenia gravis: a diagnostic breakthrough. [PDF]

Lancet Reg Health West Pac, 2023
Budhram A.
europepmc   +1 more source

A Systematic Comparison Identifies an ATP‐Based Viability Assay as Most Suitable Read‐Out for Drug Screening in Glioma Stem‐Like Cells [PDF]

, 2016
Anne Kleijn, Jenneke J. Kloezeman, Rutger K. Balvers, Mariëlle van der Kaaij, Clemens M.F. Dirven, Sieger Leenstra, Martine L.M. Lamfers   +6 more
openalex   +1 more source

In situ molecular organization and heterogeneity of the Legionella Dot/Icm T4SS

FEBS Letters, EarlyView.
We present a nearly complete in situ model of the Legionella Dot/Icm type IV secretion system, revealing its central secretion channel and identifying new components. Using cryo‐electron tomography with AI‐based modeling, our work highlights the structure, variability, and mechanism of this complex nanomachine, advancing understanding of bacterial ...
Przemysław Dutka, Yuxi Liu, Stefano Maggi, Debnath Ghosal, Jue Wang, Prashant P. Damke, Stephen D. Carter, Wei Zhao, Sukhithasri Vijayrajratnam, Joseph P. Vogel, Carrie L. Shaffer, Grant J. Jensen   +11 more
wiley   +1 more source

Mouse N2a Neuroblastoma Assay: Uncertainties and Comparison with Alternative Cell-Based Assays for Ciguatoxin Detection. [PDF]

Mar Drugs, 2023
Raposo-Garcia S, Cao A, Costas C, Louzao MC, Vilariño N, Vale C, Botana LM.   +6 more
europepmc   +1 more source

Assessing Gαq/15-signaling with IP-One: Single Plate Transfection and Assay Protocol for Cell-Based High-Throughput Assay [PDF]

, 2020
Élie Besserer‐Offroy, Rebecca L. Brouillette, Jean‐Michel Longpré, Philippe Sarret   +3 more
openalex   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Protocol for extracellular vesicle secretion-related gene screening via ExoScreen technique

STAR Protocols
Summary: Extracellular vesicles (EVs) play a key role in cancer development and cellular homeostasis by transferring the biological cargo to recipient cells. Here, we describe steps for screening EV secretion-related genes by combining a microRNA (miRNA)
Tomofumi Yamamoto, Fumihiko Urabe, Yusuke Yoshioka, Yusuke Yamamoto, Takahiro Ochiya   +4 more
doaj   +1 more source

A high-throughput microfluidic mechanoporation platform to enable intracellular delivery of cyclic peptides in cell-based assays. [PDF]

Bioeng Transl Med, 2023
Kasper SH, Otten S, Squadroni B, Orr-Terry C, Kuang Y, Mussallem L, Ge L, Yan L, Kannan S, Verma CS, Brown CJ, Johannes CW, Lane DP, Chandramohan A, Partridge AW, Roberts LR, Josien H, Therien AG, Hett EC, Howell BJ, Peier A, Ai X, Cassaday J.   +22 more
europepmc   +1 more source