Results 81 to 90 of about 88,541,361 (296)

A Protocol to Measure the Extent of Cell-to-cell Movement of RNA Viruses in Planta

Bio-Protocol, 2014
Here, we present a simple and rapid protocol to measure the extent of cell-to-cell movement of RNA viruses in planta. To do that, the green fluorescent protein (GFP) gene was incorporated into the genome of Melon necrotic spot virus (MNSV) as a coat ...
José Navarro, Marta Serra-Soriano, Vicente Pallás   +2 more
doaj   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Cucumber mosaic virus-Plant Interactions: Identification of 3a Protein Sequences Affecting Infectivity, Cell-to-Cell Movement, and Long-distance Movement

Molecular Plant-Microbe Interactions, 2001
Mutants of the Cucumber mosaic virus (CMV) movement protein (MP) were generated and analyzed for their effects on virus movement and pathogenicity in vivo.
Qiubo Li, Ki Hyun Ryu, Peter Palukaitis
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

Yeast nuclear envelope subdomains with distinct abilities to resist membrane expansion [PDF]

, 2006
Peer ...
Cohen-Fix, Orna, Campbell, Joseph L., Lorenz, Alexander, Witkin, Keren L., Hays, Thomas, Loidl, Joseph   +5 more
core   +1 more source

Genetic Analysis of the Cell-to-Cell Movement of Beet Yellows Closterovirus

Virology, 2000
A beet yellows closterovirus (BYV) variant expressing green fluorescent protein and leaves of BYV local lesion host Claytonia perfoliata were used to reveal genetic requirements for BYV cell-to-cell movement in leaf epidermis and mesophyll. A series of mutations targeting genes that are not involved in amplification of the viral positive-strand RNA was
Alzhanova, Dina V., Hagiwara, Yuka, Peremyslov, Valery V., Dolja, Valerian V.   +3 more
openaire   +2 more sources

Phosphorylation of Coat Protein by Protein Kinase CK2 Regulates Cell-to-Cell Movement of Bamboo mosaic virus Through Modulating RNA Binding

Molecular Plant-Microbe Interactions, 2014
In this study, we investigated the fine regulation of cell-to-cell movement of Bamboo mosaic virus (BaMV). We report that the coat protein (CP) of BaMV is phosphorylated in planta at position serine 241 (S241), in a process involving Nicotiana ...
Chien-Jen Hung, Ying-Wen Huang, Ming-Ru Liou, Ya-Chien Lee, Na-Sheng Lin, Menghsiao Meng, Ching-Hsiu Tsai, Chung-Chi Hu, Yau-Heiu Hsu   +8 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Hematopoietic Signaling Mechanism Revealed from a Stem/Progenitor Cell Cistrome

, 2018
Thousands of cis-elements in genomes are predicted to have vital functions. Although conservation, activity in surrogate assays, polymorphisms, and disease mutations provide functional clues, deletion from endogenous loci constitutes the gold-standard ...
Rajendran Sanalkumar, Jin Soo Kim, Chandler Zuo, Yoon-A Kang, Sunduz Keles, Prithvia Devadas, Duk Hyoung Kim, Emery H. Bresnick, Kyle J. Hewitt, Rajalekshmi Prathibha, Kirby D Johnson, Colin N. Dewey   +11 more
core   +1 more source

A live-imaging protocol to track cell movement in the Xenopus embryo

STAR Protocols, 2021
Summary: Tracking individual cell movement during development is challenging, particularly in tissues subjected to major remodeling. Currently, most live imaging techniques in Xenopus are limited to tissue explants and/or to superficial cells.
Alexandre Chuyen, Fabrice Daian, Andrea Pasini, Laurent Kodjabachian   +3 more
doaj   +1 more source