Results 51 to 60 of about 3,592,224 (262)

Remote Cell Growth Sensing Using Self-Sustained Bio-Oscillations [PDF]

open access: yes, 2018
A smart sensor system for cell culture real-time supervision is proposed, allowing for a significant reduction in human effort applied to this type of assay. The approach converts the cell culture under test into a suitable “biological” oscillator.
Daza Navarro, María Paula   +9 more
core  

A Monolithically Fabricated Combinatorial Mixer for Microchip-Based High-Throughput Cell Culturing Assays [PDF]

open access: yes, 2007
We present an integrated method to fabricate 3- D microfluidic networks and fabricated the first on-chip cell culture device with an integrated combinatorial mixer.
Ho, Dean, Liu, Mike C., Tai, Yu-Chong
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

open access: yesFEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel   +13 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

open access: yesFEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura   +2 more
wiley   +1 more source

Microvascular Endothelial Cell Culture.

open access: yesJournal of Investigative Dermatology, 1989
Microvascular endothelial cells play a central role in inflammation, tumor metastasis, and wound healing. Methods to study these processes in vitro using cells isolated from adult skin, from the inner and the outer segments of the neontal foreskin, and from experimental animals are reviewed.
openaire   +2 more sources

Cell culture

open access: yes, 2023
Published by MDPI ...
Weiskirchen, Sabine   +3 more
openaire   +2 more sources

In vitro culture of the fastidious bacteria Candidatus Liberibacter asiaticus in association with insect feeder cells [PDF]

open access: yes, 2011
Ca. Liberibacter asiaticus (LAS) is vectored by psyllids and is able to proliferate inside the insect. We therefore hypothesize that insect cells could act like feeder cells, providing nutrients in a continuous way and a favorable environment to the ...
Dollet, Michel   +3 more
core  

Development of an hydrophobic fluoro-silica surface for studying homotypic cancer cell aggregation–disaggregation as a single dynamic process in vitro [PDF]

open access: yes, 2014
Under normal conditions the detachment of anchorage-dependant cells from their extracellular matrix typically induces programmed cell death which is mediated through a pathway referred to as anoikis. However, a resistance to anoikis in cancer enables the
Arima   +48 more
core   +1 more source

Diversity and complexity in neural organoids

open access: yesFEBS Letters, EarlyView.
Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley   +1 more source

Nucleosomes indicate the in vitro radiosensitivity of irradiated bronchoepithelial and lung cancer cells [PDF]

open access: yes, 2004
Nucleosomes, which are typical cell death products, are elevated in the serum of cancer patients and are known to rapidly increase during radiotherapy.
Al-Batran SE   +14 more
core   +1 more source

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