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Germ Cell Tumor Cell Culture Techniques

2020
Optimization of cell culture protocol for a given cell line is critical for the proper conduct of in vitro experiments. Because germ cell tumors can be so heterogeneous, optimal culture conditions can vary widely between cell lines. Here, we describe our experience in routine culture and cryopreservation of germ cell tumor cell culture.
John T, Lafin, James F, Amatruda, Aditya, Bagrodia   +2 more
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Liver Cell Culture Techniques

2009
Different sources of hepatic tissue, including whole or split livers from organ donors or from cadavers, waste liver from therapeutic hepatectomies or small-sized surgical biopsies, can be successfully used to prepare human hepatocytes cultures.
Castell, Jose V, Gomez-Lechon, Maria Jose   +1 more
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Direct microcalorimetry as a technique in cell cultures

Baillière's Clinical Endocrinology and Metabolism, 1997
In vitro studies of energy metabolism in isolated cells contribute to improved knowledge of human energy metabolism under normal and pathological conditions. In every cellular system energy is taken up, metabolized and finally transformed into heat, which is dissipated into the environment.
H, Böttcher, P, Fürst
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Mammalian Cell Tissue Culture Techniques

Current Protocols in Molecular Biology, 2016
AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with
Katy, Phelan, Kristin M, May
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Reagents and Techniques for Cell Culture

Manuals in Biomedical Research, 2010
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Aseptic Technique for Cell Culture

Current Protocols in Cell Biology, 1998
AbstractThis unit describes some of the ways that a laboratory can deal with the constant threat of microbial contamination in cell cultures. A protocol on aseptic technique is described first. This catch‐all term universally appears in any set of instructions pertaining to procedures in which noncontaminating conditions must be maintained. In reality,
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Basic Techniques in Mammalian Cell Tissue Culture

Current Protocols in Toxicology, 2015
AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy, Phelan, Kristin M, May
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Essential Techniques of Cancer Cell Culture

2003
A primary cell culture is the initial culture set up directly from a body tissue. Primary cancer cultures can be initiated and derived from a variety of tissue types such as solid tumor fragments (primary or metastatic) or cell suspensions, for example, aspirates, including peritoneal ascites or pleural effusions.
Kenneth G, MacLeod, Simon P, Langdon
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Cell Culture Techniques

2011
1. Guidance on Good Cell Culture Practice (GCCP) Anna Bal-Price and Sandra Coecke 2. Induced Pluripotent Stem Cells (iPSCs): An Emerging Model System for the Study of Human Neurotoxicology M. Diana Neely, Andrew M. Tidball, Asad A. Aboud, Kevin C. Ess, and Aaron B. Bowman 3. Neural Stem Cells Roshan Tofighi, Christoffer Tamm, Michaela Moors, and Sandra
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Techniques for Mammalian Cell Tissue Culture

Current Protocols in Human Genetics, 1994
AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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