Results 131 to 140 of about 12,578,619 (347)

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Cytotoxic effects of Pseudocerastes persicus venom and its HPLC fractions on lung cancer cells

, 2019
Benyamin Shahbazi, Zahra Salehi-Najafabadi, Hamidreza Goudarzi, Mahnaz Sajadi, Fatemeh Tahoori, Masoumeh Bagheri   +5 more
openalex   +1 more source

Evaluation of anti-inflammatory potential of extract, fractions and major compounds of Ponciri Fructus in LPS-induced RAW 264.7 cells

, 2023
Gopal Lamichhane, Prakash Raj Pandeya, Ramakanta Lamichhane, Hong Dah Yun, Amit Kumar Shrivastava, J. Cheon, Biswash Sapkota, Hari Prasad Devkota, Hyun‐Ju Jung   +8 more
openalex   +1 more source

Methods to separate nuclear soluble fractions reflecting localizations in living cells [PDF]

, 2021
Yutaka Ogawa, Naoko Imamoto
openalex   +1 more source

Keratin 19 as a prognostic marker and contributing factor of metastasis and chemoresistance in high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Keratin 19 (KRT19) is overexpressed in high‐grade serous ovarian cancer with high levels of Kallikrein‐related peptidases (KLK) 4–7 and is associated with poor survival. In vivo analyses demonstrate that elevated KRT19 increases peritoneal tumour burden.
Sophia Bielesch, Isabel Vogel, Sina Nokodian, Johanna Moeller, Antonia Blechschmidt, Vicky Hecht, Vanessa Kuentzel, Katharina Thiedig, Melissa Schwab, Oliver Schilling, Holger Bronger, Marion Kiechle, Viktor Magdolen, Tobias Dreyer   +13 more
wiley   +1 more source

Rethinking plastic waste: innovations in enzymatic breakdown of oil‐based polyesters and bioplastics

FEBS Open Bio, EarlyView.
Plastic pollution remains a critical environmental challenge, and current mechanical and chemical recycling methods are insufficient to achieve a fully circular economy. This review highlights recent breakthroughs in the enzymatic depolymerization of both oil‐derived polyesters and bioplastics, including high‐throughput protein engineering, de novo ...
Elena Rosini, Nicolò Antonelli, Gianluca Molla   +2 more
wiley   +1 more source

Identification of β-catenin-interacting proteins in nuclear fractions of native rat collecting duct cells [PDF]

, 2017
Jacqueline R. Hwang, Chung‐Lin Chou, Barbara Medvar, Mark A. Knepper, Hyun Jun Jung   +4 more
openalex   +1 more source

Dietary nitrate and nitrite protect against doxorubicin‐induced cardiac fibrosis and oxidative protein damage in tumor‐bearing mice

FEBS Open Bio, EarlyView.
Chemotherapies such as doxorubicin can have toxic effects on healthy cardiovascular/heart tissue. Following up on a doxorubicin toxicity study in mice without tumors where nitrate water was cardioprotective (lessened toxicity), this study with tumor‐bearing mice undergoing doxorubicin treatment showed no negative effect of nitrate and nitrite on drug ...
Rama D. Yammani, Xiaofei Chen, Nildris Cruz‐Diaz, Xuewei Zhu, Swati Basu, Daniel B. Kim‐Shapiro, David R. Soto‐Pantoja, Leslie B. Poole   +7 more
wiley   +1 more source

Transcriptomic, Proteomic, and Genomic Mutational Fraction Differences Based on HPV Status Observed in Patient-Derived Xenograft Models of Penile Squamous Cell Carcinoma [PDF]

Niki M. Zacharias, Luis Segarra, Keiko Akagi, Natalie W. Fowlkes, Huiqin Chen, Angelita Alaniz, Carolyn De La Cerda, Pedro I. Pesquera, Yuanxin Xi, Jing Wang, Jad Chahoud, Xin Lü, Priya Rao, Magaly Martínez‐Ferrer, Curtis A. Pettaway   +14 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source