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State of the art in cell-cell fusion. [PDF]
Methods in molecular biology, 2015 Mammalian life begins with a cell-cell fusion event, i.e. the fusion of the spermatozoid with the oocyte and needs further cell-cell fusion processes for the development, growth, and maintenance of tissues and organs over the whole life span. Furthermore, cellular fusion plays a role in infection, cancer, and stem cell-dependent regeneration as well as
L. Willkomm, W. Bloch
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Fusion of Tumour Cells with Host Cells [PDF]
Nature New Biology, 1972 THE A9 cell is an 8-azaguanine-resistant derivative of the L cell line1. It lacks the enzyme inosinic acid pyrophosphorylase and is thus unable to grow in media such as HAT2 in which endogenous synthesis of nucleic acid is blocked by aminopterin. The A9 line has little ability to grow progressively in vivo.
Wiener, F, Fenyo, E, Klein, G, Harris, H
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Somatic Cell Genetics, 1977
Hybrid cells were prepared by fusin an immunoglobulin-secreting mouse myeloma lin e (B cell) with an allogenic T-cell lymphoma which expresses the surface antigen Thy 1. The resulting hybrids expressed H2 antigens of both parental cells and secreted the immunoblobulin of the myeloma parent but did not express the Thy 1 antigen of the lymphoma parent ...
Kohler, G, Pearson, T, Milstein, C
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Hybrid cells were prepared by fusin an immunoglobulin-secreting mouse myeloma lin e (B cell) with an allogenic T-cell lymphoma which expresses the surface antigen Thy 1. The resulting hybrids expressed H2 antigens of both parental cells and secreted the immunoblobulin of the myeloma parent but did not express the Thy 1 antigen of the lymphoma parent ...
Kohler, G, Pearson, T, Milstein, C
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The mechanism of cell-cell fusion
Trends in Biochemical Sciences, 1979Abstract Three stages are involved in cell-cell fission mediated by viruses and chemicals: (1) agglutination; (2) membrane fusion between adjacent cells; and (3) osmotic swelling. The recogni tion that stages (2) and (3) are separate events has led to a better understanding of the overall process.
Charles A. Pasternak, S. Knutton
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Science, 2012
Cell Biology![Figure][1] CREDIT: TSUKASA OIKAWA Osteoclasts are cells that promote bone remodeling, and their hyperactivity is linked to bone-destructive disorders, including osteoporosis. Activated osteoclast precursors develop columnar actin structures, known as podosomes, which are similar to the invadopodia observed in invasive cancer cells ...
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Cell Biology![Figure][1] CREDIT: TSUKASA OIKAWA Osteoclasts are cells that promote bone remodeling, and their hyperactivity is linked to bone-destructive disorders, including osteoporosis. Activated osteoclast precursors develop columnar actin structures, known as podosomes, which are similar to the invadopodia observed in invasive cancer cells ...
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Nature, 1975
WE report new findings on the chemically-induced fusion of hen erythrocytes and discuss possible ways in which the lipid and protein/glycoprotein components of membranes may behave during the process of membrane fusion. Our observations are consistent with Poste and Allison's1 idea that the aggregation of intrinsic membrane proteins is important in ...
Jack A. Lucy +3 more
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WE report new findings on the chemically-induced fusion of hen erythrocytes and discuss possible ways in which the lipid and protein/glycoprotein components of membranes may behave during the process of membrane fusion. Our observations are consistent with Poste and Allison's1 idea that the aggregation of intrinsic membrane proteins is important in ...
Jack A. Lucy +3 more
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Methodologies in the Study of Cell–Cell Fusion
Methods, 1998The process of membrane fusion has been profitably studied by fusing cells that express fusion proteins on their surfaces to the membranes of target cells. Primary methods for monitoring the occurrence of fusion between cells are measurement of formation of heterokaryons, measurement of activation of reporter genes, measurement of transfer of lipidic ...
Grigory B. Melikyan, Fredric S. Cohen
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Unveiling the Mechanisms of Cell-Cell Fusion
Science, 2005Cell-cell fusion is fundamental to the development and physiology of multicellular organisms, but little is known of its mechanistic underpinnings. Recent studies have revealed that many proteins involved in cell-cell fusion are also required for seemingly unrelated cellular processes such as phagocytosis, cell migration, axon growth, and ...
Eric N. Olson +2 more
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Nature, 1970
Lysolecithin will induce cell fusion in several different types of cell, and both multinucleated syncytia and heterokaryons can be formed.
A. R. Poole, J. A. Lucy, J. I. Howell
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Lysolecithin will induce cell fusion in several different types of cell, and both multinucleated syncytia and heterokaryons can be formed.
A. R. Poole, J. A. Lucy, J. I. Howell
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