Results 1 to 10 of about 2,659,061 (219)

A novel in vitro system of supported planar endosomal membranes (SPEMs) reveals an enhancing role for cathepsin B in the final stage of Ebola virus fusion and entry

Microbiology Spectrum, 2023
Ebola virus (EBOV) causes a hemorrhagic fever with fatality rates up to 90%. The EBOV entry process is complex and incompletely understood. Following attachment to host cells, EBOV is trafficked to late endosomes/lysosomes where its glycoprotein (GP) is ...
Laura Odongo, Betelihem H. Habtegebrael, Volker Kiessling, Judith M. White, Lukas K. Tamm   +4 more
doaj   +1 more source

Reproducible reformation of a bilayer lipid membrane using microair bubbles

Droplet, 2023
Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic
Izumi Hashimoto, Toshihisa Osaki, Hirotaka Sugiura, Hisatoshi Mimura, Sho Takamori, Norihisa Miki, Shoji Takeuchi   +6 more
doaj   +1 more source

Hydrophilic nanoparticles that kill bacteria while sparing mammalian cells reveal the antibiotic role of nanostructures

Nature Communications, 2022
Developing antibacterial agents which don’t have cytotoxic effects against mammalian cells is of interest for biomedical applications. Here, the authors explore how attaching inert polymer brushes to different sized nanoparticles can result in toxicity ...
Yunjiang Jiang, Wan Zheng, Keith Tran, Elizabeth Kamilar, Jitender Bariwal, Hairong Ma, Hongjun Liang   +6 more
doaj   +1 more source

Determining a critical threshold for G6PD activity below which red blood cell response to oxidative stress is poor

Malaria Journal, 2020
Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most common enzyme disorder in the world. Its main function is to generate NADPH that is required for anti-oxidative pathway in the cells especially in red blood cells (RBC).
Maria Swastika, Alida R. Harahap, Lydia V. Panggalo, Sri Widia A. Jusman, Ari W. Satyagraha   +4 more
doaj   +1 more source

Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution

Micromachines, 2021
This paper describes a method for a bilayer lipid membrane (BLM) formation using a perforated sheet along with an open chamber. Microscopic observation of the formed membrane showed a typical droplet interface bilayer.
Nobuo Misawa, Satoshi Fujii, Koki Kamiya, Toshihisa Osaki, Shoji Takeuchi   +4 more
doaj   +1 more source

RNAi screening reveals a large signaling network controlling the Golgi apparatus in human cells

Molecular Systems Biology, 2012
The Golgi apparatus has many important physiological functions, including sorting of secretory cargo and biosynthesis of complex glycans. These functions depend on the intricate and compartmentalized organization of the Golgi apparatus.
Joanne Chia, Germaine Goh, Victor Racine, Susanne Ng, Pankaj Kumar, Frederic Bard   +5 more
doaj   +1 more source

Drug resistance-associated changes in sphingolipids and ABC transporters occur in different regions of membrane domains

Journal of Lipid Research, 2005
We have recently shown that two ATP binding cassette (ABC) transporters are enriched in Lubrol-resistant noncaveolar membrane domains in multidrug-resistant human cancer cells [Hinrichs, J. W. J., K. Klappe, I. Hummel, and J. W. Kok. 2004.
John W.J. Hinrichs, Karin Klappe, Manon van Riezen, Jan W. Kok   +3 more
doaj   +1 more source

Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening

Nature Communications, 2016
Establishment and maintenance of apico-basal polarity in epithelial organs needs to be tightly coupled with cell division. Here the authors show that the Rab35 GTPase tethers intracellular vesicles containing key apical determinants at the cleavage site,
Kerstin Klinkert, Murielle Rocancourt, Anne Houdusse, Arnaud Echard   +3 more
doaj   +1 more source

In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties

Nature Communications, 2019
Regulated exocytosis of neuronal synaptic vesicles is substantially faster than that of endocrine dense core vesicles despite similar molecular machineries.
Alex J. B. Kreutzberger, Volker Kiessling, Christopher Stroupe, Binyong Liang, Julia Preobraschenski, Marcelo Ganzella, Mark A. B. Kreutzberger, Robert Nakamoto, Reinhard Jahn, J. David Castle, Lukas K. Tamm   +10 more
doaj   +1 more source

Membrane dynamics and cell polarity: the role of sphingolipids

Journal of Lipid Research, 2003
In recent years, glycosphingolipids (GSLs) have attracted widespread attention due to the appreciation that this class of lipids has a major impact on biological life.
Dick Hoekstra, Olaf Maier, Johanna M. van der Wouden, Tounsia Aït Slimane, Sven C.D. van IJzendoorn   +4 more
doaj   +1 more source