Results 11 to 20 of about 3,918,856 (298)

Electroporation of cell membranes [PDF]

Biophysical Journal, 1989
Electric pulses of intensity in kilovolts per centimeter and of duration in microseconds to milliseconds cause a temporary loss of the semipermeability of cell membranes, thus leading to ion leakage, escape of metabolites, and increased uptake by cells of drugs, molecular probes, and DNA.
openaire   +2 more sources

Human hepatic cell behavior on polysulfone membrane with double porosity level [PDF]

, 2013
In the membrane-based bioartificial livers developed up to now,the hepatic cells were located either in the fibers lumen or in the cartridge,with limited capacity for cell hosting.Here,we designed a polysulfone (PSU) membrane with a double porosity ...
Adrianus, Gerard Nathanael, Aimar, Pierre, Bacchin, Patrice, Cerino, Giulia, Dufresne, Murielle, Legallais, Cécile, Remigy, Jean-Christophe   +6 more
core   +2 more sources

Automated Segmentation of Cells with IHC Membrane Staining [PDF]

, 2011
This study presents a fully automated membrane segmentation technique for immunohistochemical tissue images with membrane staining, which is a critical task in computerized immunohistochemistry (IHC).
Acquaviva, Andrea, Di Cataldo, Santa, Ficarra, Elisa, Macii, Enrico   +3 more
core   +1 more source

Rab35 GTPase couples cell division with initiation of epithelial apico-basal polarity and lumen opening

Nature Communications, 2016
Establishment and maintenance of apico-basal polarity in epithelial organs needs to be tightly coupled with cell division. Here the authors show that the Rab35 GTPase tethers intracellular vesicles containing key apical determinants at the cleavage site,
Kerstin Klinkert, Murielle Rocancourt, Anne Houdusse, Arnaud Echard   +3 more
doaj   +1 more source

In vitro fusion of single synaptic and dense core vesicles reproduces key physiological properties

Nature Communications, 2019
Regulated exocytosis of neuronal synaptic vesicles is substantially faster than that of endocrine dense core vesicles despite similar molecular machineries.
Alex J. B. Kreutzberger, Volker Kiessling, Christopher Stroupe, Binyong Liang, Julia Preobraschenski, Marcelo Ganzella, Mark A. B. Kreutzberger, Robert Nakamoto, Reinhard Jahn, J. David Castle, Lukas K. Tamm   +10 more
doaj   +1 more source

Membrane dynamics and cell polarity: the role of sphingolipids

Journal of Lipid Research, 2003
In recent years, glycosphingolipids (GSLs) have attracted widespread attention due to the appreciation that this class of lipids has a major impact on biological life.
Dick Hoekstra, Olaf Maier, Johanna M. van der Wouden, Tounsia Aït Slimane, Sven C.D. van IJzendoorn   +4 more
doaj   +1 more source

A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange

Micromachines, 2020
Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection.
Yoshihisa Ito, Yusuke Izawa, Toshihisa Osaki, Koki Kamiya, Nobuo Misawa, Satoshi Fujii, Hisatoshi Mimura, Norihisa Miki, Shoji Takeuchi   +8 more
doaj   +1 more source

TPLATE recruitment reveals endocytic dynamics at sites of symbiotic interface assembly in arbuscular mycorrhizal interactions [PDF]

, 2019
Introduction: Arbuscular mycorrhizal (AM) symbiosis between soil fungi and the majority of plants is based on a mutualistic exchange of organic and inorganic nutrients.
Bonfante, Paola, Carotenuto, Gennaro, Chabaud, Mireille, Chiapello, Marco, Faccio, Antonella, Fiorilli, Valentina, Genre, Andrea, Russo, Giulia, Van Damme, Daniël, Volpe, Veronica   +9 more
core   +3 more sources

Mechanics and regulation of cytokinetic abscission

Frontiers in Cell and Developmental Biology, 2022
Cytokinetic abscission leads to the physical cut of the intercellular bridge (ICB) connecting the daughter cells and concludes cell division. In different animal cells, it is well established that the ESCRT-III machinery is responsible for the ...
Virginia Andrade, Virginia Andrade, Arnaud Echard   +2 more
doaj   +1 more source

An ultra scale-down analysis of the recovery by dead-end centrifugation of human cells for therapy. [PDF]

, 2014
An ultra scale-down method is described to determine the response of cells to recovery by dead-end (batch) centrifugation under commercially defined manufacturing conditions.
Delahaye, M, Hoare, M, Lawrence, K, Ward, SJ   +3 more
core   +1 more source