Results 91 to 100 of about 13,330,610 (394)

Fast Mechanically Driven Daughter Cell Separation Is Widespread in Actinobacteria

mBio, 2016
Dividing cells of the coccoid Gram-positive bacterium Staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (DCS) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically ...
Xiaoxue Zhou, David K. Halladin, J. Theriot   +2 more
semanticscholar   +1 more source

Transcript-indexed ATAC-seq for precision immune profiling. [PDF]

, 2018
T cells create vast amounts of diversity in the genes that encode their T cell receptors (TCRs), which enables individual clones to recognize specific peptide-major histocompatibility complex (MHC) ligands. Here we combined sequencing of the TCR-encoding
Buenrostro, Jason D, Chang, Howard Y, Corces, M Ryan, Davis, Mark M, Gennert, David G, Granja, Jeffrey M, Greenleaf, William J, Khavari, Paul A, Khodadoust, Michael S, Kim, Youn H, Lareau, Caleb A, Li, Rui, Mumbach, Maxwell R, Parker, Kevin R, Qi, Yanyan, Rubin, Adam J, Saligrama, Naresha, Satpathy, Ansuman T, Schep, Alicia N, Serratelli, William S, Wei, Yuning, Wu, Beijing   +21 more
core   +1 more source

Ergothioneine supplementation improves pup phenotype and survival in a murine model of spinal muscular atrophy

FEBS Letters, EarlyView.
Spinal muscular atrophy (SMA) is a genetic disease affecting motor neurons. Individuals with SMA experience mitochondrial dysfunction and oxidative stress. The aim of the study was to investigate the effect of an antioxidant and neuroprotective substance, ergothioneine (ERGO), on an SMNΔ7 mouse model of SMA.
Francesca Cadile, Daniela Ratto, Giorgia Rastelli, Ottavia Eleonora Ferraro, Caterina Temporini, Sunil Kumar, Simona Boncompagni, Paola Rossi, Monica Canepari   +8 more
wiley   +1 more source

Modelling and prototyping of a microfluidic cartridge for extracting immune cells from small volume blood samples

Current Directions in Biomedical Engineering, 2020
Within the human blood immune cells assume specific functions to modulate and control the human immune response. Consequently, they are used for the diagnosis and treatment of autoimmune and hematologic diseases.
Behrens Stephan, Sonntag Frank, Schmieder Florian   +2 more
doaj   +1 more source

Counterflow dielectrophoresis for trypanosome enrichment and detection in blood [PDF]

, 2012
Human African trypanosomiasis or sleeping sickness is a deadly disease endemic in sub-Saharan Africa, caused by single-celled protozoan parasites. Although it has been targeted for elimination by 2020, this will only be realized if diagnosis can be ...
A Carlsson, F Chappuis, FH Labeed, J Vykoukal, JA Rodriguez, KS Ralston, M Jacob, M Murray, MP Barrett, NG Green, P Gascoyne, SC Welburn, SH Holm, U Kim, WH Lumsden, WT Freeman, X-B Wang, XB Wang, Y Huang, ZK Njiru   +19 more
core   +1 more source

Amidase Activity of AmiC Controls Cell Separation and Stem Peptide Release and Is Enhanced by NlpD in Neisseria gonorrhoeae*

Journal of Biological Chemistry, 2016
The human-restricted pathogen Neisseria gonorrhoeae encodes a single N-acetylmuramyl-l-alanine amidase involved in cell separation (AmiC), as compared with three largely redundant cell separation amidases found in Escherichia coli (AmiA, AmiB, and AmiC).
J. Lenz, Elizabeth A. Stohl, Rosanna M. Robertson, K. Hackett, K. Fisher, K. Xiong, Mijoon Lee, D. Hesek, S. Mobashery, H. Steven Seifert, C. Davies, J. P. Dillard   +11 more
semanticscholar   +1 more source

Microbial exopolysaccharide production by polyextremophiles in the adaptation to multiple extremes

FEBS Letters, EarlyView.
Polyextremophiles are microorganisms that endure multiple extreme conditions by various adaptation strategies that also include the production of exopolysaccharides (EPSs). This review provides an integrated perspective on EPS biosynthesis, function, and regulation in these organisms, emphasizing their critical role in survival and highlighting their ...
Tracey M Gloster, Ebru Toksoy Öner
wiley   +1 more source

Integrated acoustic and magnetic separation in microfluidic channels [PDF]

, 2009
With a growing number of cell-based biotechnological applications, there is a need for particle separation systems capable of multiparameter separations at high purity and throughput, beyond what is presently offered by traditional methods including ...
H. Tom Soh, Henrik Bruus, Jonathan D. Adams, Patrick Thévoz, Yosioka K.   +4 more
core   +2 more sources

Single cis‐elements in brassinosteroid‐induced upregulated genes are insufficient to recruit both redox states of the BIL1/BZR1 DNA‐binding domain

FEBS Letters, EarlyView.
Phytohormone brassinosteroid‐induced gene regulation by the transcription factor BIL1/BZR1 involves redox‐dependent DNA‐binding alternation and interaction with the transcription factor PIF4. The reduced BIL1/BZR1 dimer binds preferred cis‐elements, while oxidation alters its oligomerization state and disrupts DNA‐binding ability.
Shohei Nosaki, Masae Ohtsuka, Takeshi Nakano, Masaru Tanokura, Takuya Miyakawa   +4 more
wiley   +1 more source

3D Printed Devices for the Separation of Blood Plasma from Capillary Samples

Micromachines
Sample preparation is a critical requirement for many clinical tests and diagnostic procedures, but it is difficult to perform on a lab-on-a-chip platform.
Giulia Deiana, Stewart Smith
doaj   +1 more source