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Methods in Cell Separations

2007
Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented.
Bo Mattiasson   +4 more
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Immunomagnetic Cell Separation

2003
In metastasis research, it may sometimes be necessary to separate populations of tumor cells from a mixed cell population such as a tumor, peripheral blood, or bone marrow. In addition, the normal counterparts of populations of tumor cells can be separated to allow direct comparisons to be made (1).In recent years magnetic bead separation techniques ...
Susan Davies, Catherine F. Clarke
openaire   +3 more sources

To separate cells

Trends in Biotechnology, 1998
Abstract Cell Separation Methods and Applications edited by D. Recktenwald and A. Radbruch, Marcel Dekker, 1998.
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Electrophoretic separation of cells

Journal of Chromatography B: Biomedical Sciences and Applications, 1987
There are several kinds of cell electrophoresis. The most important are free-flow electrophoresis, scaled-up free-flow electrophoresis and column electrophoresis. All kinds of cell electrophoresis, especially free-flow electrophoresis, have been improved to a very high standard of separation accuracy, and their application possibilities are extended ...
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Cell Separation

1999
Publisher Summary This chapter discusses cell separation in detail. The aim of cell separation is to either positively select a target cell type (enrichment) or remove an unwanted cell type (depletion). The chapter presents a review of cell-separation methods and includes an analysis of their physical basis and performance.
Robert E. Nordon, Klaus Schindhelm
openaire   +2 more sources

Dielectrophoretic separation of living cells

Canadian Journal of Microbiology, 1971
Dielectrophoretic separation studies were undertaken to investigate the possibility of separating cells with varying physiological character. An attempt was made to define the importance of size in the determination of separability. Living cells of Saccharomyces cerevisiae var. ellipsoideus were separated from the same cells killed by heating, using a
B. D. Mason, P. M. Townsley
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Cell separation in the buffy coat

Biorheology, 1988
One of the most rapid methods to determine cell counts in whole blood is by way of layer thickness measurements of the buffy coat. The purpose of this study was to determine the separation and purity of blood cells in the different layers of the buffy coat.
Peter C. Chen   +2 more
openaire   +2 more sources

Fundamentals of affinity cell separations

ELECTROPHORESIS, 2017
AbstractCell separations using affinity methods continue to be an enabling science for a wide variety of applications. In this review, we discuss the fundamental aspects of affinity separation, including the competing forces for cell capture and elution, cell‐surface interactions, and models for cell adhesion.
Ye Zhang, Veronica Lyons, Dimitri Pappas
openaire   +3 more sources

Phase separation in necrotic cells

Biochemical and Biophysical Research Communications, 2017
Necrotic cells are known to develop characteristic membrane blebs. We measured protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? 2.
Priyanka S. Rana   +5 more
openaire   +3 more sources

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