Results 61 to 70 of about 6,529,508 (347)
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Proof-of-Concept of a Novel Cell Separation Technology Using Magnetic Agarose-Based Beads
Magnetochemistry, 2022 The safety of the cells used for Advanced Therapy Medicinal Products is crucial for patients. Reliable methods for the cell purification are very important for the commercialization of those new therapies.
Nils A. Brechmann +6 more
doaj +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion [PDF]
, 2000 Duplicating centrosomes are paired during interphase, but are separated at the onset of mitosis. Although the mechanisms controlling centrosome cohesion and separation are important for centrosome function throughout the cell cycle, they remain poorly ...
Fry, A. M. +9 more
core +1 more source
Diversity and complexity in neural organoids
FEBS Letters, EarlyView.Neural organoid research aims to expand genetic diversity on one side and increase tissue complexity on the other. Chimeroids integrate multiple donor genomes within single organoids. Self‐organising multi‐identity organoids, exogenous cell seeding, or enforced assembly of region‐specific organoids contribute to tissue complexity.
Ilaria Chiaradia, Madeline A. Lancaster
wiley +1 more source
Cell Migration with Multiple Pseudopodia: Temporal and Spatial Sensing Models [PDF]
, 2013 Cell migration triggered by pseudopodia (or “false feet”) is the most used method of locomotion. A 3D finite element model of a cell migrating over a 2D substrate is proposed, with a particular focus on the mechanical aspects of the biological phenomenon.
ALLENA, Rachele
core +1 more source
Development of a microfluidic setup for the isolation and imaging of immune cells
Current Directions in Biomedical EngineeringSpecialised cells in the blood play an important role in controlling the immune responses. Thus, these cells are also valuable for the diagnosis and treatment of diseases such as autoimmune disorders and cancer.
Petzold Mandy +4 more
doaj +1 more source
Biomedical Applications of Polymeric Cryogels
Applied Sciences, 2019 The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over
Monireh Bakhshpour +3 more
doaj +1 more source
An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Flow Separation in Rocket Nozzles, a Simple Criteria [PDF]
, 2005 Cold and hot flow tests were conducted to investigate the flow separation in rocket nozzles. The results are presented. A separation data base including a wide range of literature data is established to evaluate the influence of propellant combination ...
Ralf Stark, Stark, Ralf
core +1 more source