Results 71 to 80 of about 237,743 (307)

Beta-sheet containment by flanking prolines: molecular dynamic simulations of the inhibition of beta-sheet elongation by proline residues in human prion protein.

, 2007
Previous molecular dynamic simulations have reported elongation of the existing β-sheet in prion proteins. Detailed examination has shown that these elongations do not extend beyond the proline residues flanking these β-sheets.
Shamsir, M.S., Dalby, A.R.
core   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Keratinocyte cytoskeletal roles in cell sheet engineering

, 2013
Background: There is an increasing need to understand cell-cell interactions for cell and tissue engineering purposes, such as optimizing cell sheet constructs, as well as for examining adhesion defect diseases.
Min Ye Shen, Shen, Min Ye, Qi Wei, Daniel Reidler, Wei, Qi, Hayden Huang, Reidler, Daniel, Huang, Hayden   +7 more
core   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

Bioengineered periosteal progenitor cell sheets to enhance tendon-bone healing in a bone tunnel

Biomedical Journal, 2012
Background: Tendon-bone tunnel healing is crucial for long term success in anterior cruciate liga­ment (ACL) reconstruction. The periosteum contains osteochondral progenitor cells that can differenti­ate into osteoblasts and chondroblasts during tendon ...
Chih-Hsiang Chang, Chih-Hwa Chen, Hsia-Wei Liu, Shu-Wen Whu, Shih-Hui Chen, Ching-Lin Tsai, Ging-Ho Hsiue   +6 more
doaj   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy

, 2016
Membrane remodeling is an essential part of transferring components to and from the cell surface and membrane-bound organelles and for changes in cell shape, which are particularly critical during cell division.
Kirchhausen, Tom, Skillern, Wesley, Betzig, Eric, Chen, Bi-Chang, Chou, Yi-ying, Upadhyayula, Srigokul, Aguet, François, Marino, Eric, Danuser, Gaudenz, Liu, Tsung-Li, Findlay, Greg, Pasham, Mithun, Findlay, Greg,, Gaudin, Raphaël, Megason, Sean, Cocucci, Emanuele, Legant, Wesley,, Mosaliganti, Kishore, He, Kangmin, Legant, Wesley R.   +19 more
core   +1 more source

Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly [PDF]

, 2012
Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells ...
Barbara Müller, Viola Baumgärtel, Müller, Barbara, Lamb, Don C., Baumgärtel, Viola, Don C. Lamb   +5 more
core   +1 more source