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The cell surface charge of Bodo sp. (Kinetoplastida: Bodonidae)

European Journal of Protistology, 1993
Cytochemistry, using cationized ferritin particles at pH 7.2 and colloidal iron particles at pH 1.8, and cell electrophoresis were used to analyze the surface charge of the protozoan Bodo sp. Surface anionic sites are homogeneously distributed throughout the protozoan surface, including the flagella, the cytostome and the flagellar pocket.
Marcia Attias, Wanderley De Souza
exaly   +3 more sources

Probing cell surface charge by scanning electrode potential

Electrophoresis, 2002
A static mercury electrode was used for measurement of double-layer charge displacement signals caused by living plant cells of the unicellular marine alga Dunaliella tertiolecta. By scanning the electrode potential a point is reached where the charge density of a plant cell compensates the electrode charge density. The experimentally determined values
Vesna Svetličič, Amela Hožič
exaly   +4 more sources

Positively charged groups at cell surfaces

International Journal of Cancer, 1968
AbstractAn attempt was made to identify positively charged amino‐groups at the electrokinetic surfaces of human and mouse erythrocytes, cultured cells derived from a human osteogenic sarcoma, and murine ascites sarcoma 37 cells. Following reactions of these cells with formaldehyde, 2,4,6‐trinitrobenzenesulfonic acid; 2‐chloro‐3,5‐dinitropyridine or 2 ...
L, Weiss, J, Bello, T L, Cudney
openaire   +2 more sources

Surface charge of protoplasts and their significance in cell-cell interaction

Planta, 1978
ζ-potential of mesophyll protoplasts of tobacco (Nicotiana tabacum L.), petunia (Petunia hybrida Hort.), turnip (Brassica rapa L.) and cowpea (Vigna unguiculata L. Walpers) was determined by use of a cell electrophoresis apparatus. All protoplasts examined showed a constant negative value of-10 to-35 mV.
T, Nagata, G, Melchers
openaire   +2 more sources

Cell-surface hydrophobicity and cell-surface charge of Azospirillum spp.

FEMS Microbiology Ecology, 2006
The cell-surface charge and cell-surface hydrophobicity of 10 strains of Azospirillum spp. were evaluated by the aqueous, two-polymer phase partitioning assay. Azospirillum spp. had moderate cell-surface hydrophobicity and charge, lower than known values for human pathogens. The hydrophobicity and charge values were higher in solid than in liquid media.
Thelma Castellanos   +2 more
openaire   +1 more source

Determination of cell surface charge by photometric titration

Journal of Biochemical and Biophysical Methods, 1997
A colloid titration method has been frequently used to determine the number of charged residues at the cell surface. Here we present a new version of this technique, based on photometric measurements of a metachromatic shift in the maximum absorption of toluidine blue as it binds to the cell surface.
K, Thethi   +5 more
openaire   +2 more sources

Electrophoresis of cells and the biological relevance of surface charge

ELECTROPHORESIS, 2002
Recent developments in electrophoresis of cells are reviewed. Problems and progress in automation and miniaturization of analytical electrophoresis instruments as well as in the interpretation of experimentally determined electrophoretic mobility (EPM) data are summarized: In recent times, the EPM determination techniques not only became more reliable ...
Mehrishi, J., Bauer, J.
openaire   +3 more sources

Cell surface charge. Correlation of partition with electrophoresis

Biochimica et Biophysica Acta (BBA) - General Subjects, 1979
Critical mixtures of aqueous solutions of polymers separate into two or more immiscible phases. Particulate materials distribute in such phase systems generally between one bulk phase and the interface between bulk phases. The distribution is described by a simple partition law, and is quantitatively determined by, inter alia, the nature of the ...
C M, Ballard   +2 more
openaire   +2 more sources

The Surface Charge of Cells Producing hCG

1984
The cell surface is the interface between the cell and its environment. The surface acts to regulate cell function through antigens, receptors, cell-cell interaction, and other factors. One important and measurable property of the cell surface is charge.
L L, Hause, N, Tosaka, R A, Pattillo
openaire   +2 more sources

Interaction of polycations with cell-surface negative charges of epithelial cells

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1992
The interaction between various polycations and cultured glomerular epithelial cells was studied by cell electrophoresis. It was shown that the glomerular epithelial cell presents a negatively charged surface which imparts a zeta potential of -29.0 +/- 1.5 mV at the peripheral layer of the plasma membrane.
A K, Singh, B S, Kasinath, E J, Lewis
openaire   +2 more sources

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