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Expression of a Fungal Cellobiohydrolase in Insect Cells
Biochemical and Biophysical Research Communications, 1997The gene for Trichoderma reesei cellobiohydrolase I (CBHI) was expressed with a recombinant baculovirus and high levels of secreted protein were produced in Spodoptera frugiperda and Trichoplusia ni insect cells. Electrophoretic analysis indicated that the recombinant CBHI (rCBHI) was similar in apparent molecular weight to the native form and ...
Teeri, Tuula +4 more
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Journal of Molecular Biology, 2013
Cellulases, glycoside hydrolases that catalyze the degradation of cellulose, are classified as either endoglucanases or cellobiohydrolases (CBHs) based on their architecture and mode of action on the cellulose. CBHs bind the cellulose chain in a more or less closed tunnel and cleave off cellobiose units processively from one end of the cellulosic ...
Mats, Sandgren +7 more
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Cellulases, glycoside hydrolases that catalyze the degradation of cellulose, are classified as either endoglucanases or cellobiohydrolases (CBHs) based on their architecture and mode of action on the cellulose. CBHs bind the cellulose chain in a more or less closed tunnel and cleave off cellobiose units processively from one end of the cellulosic ...
Mats, Sandgren +7 more
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Cellobiohydrolase (CBH) Activity Assays
2018Cellulosic biomass is the most abundant biopolymer on the earth. It has great potential to quench the thirst of liquid energy by producing biofuels and thus help to mitigate human reliance on fossil fuels. Although several cellulase activity assay methods have been used to disintegrate the glycosidic bonds, the appropriate selection of substrates and ...
Hem Kanta, Sharma +2 more
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A biochemical comparison of fungal GH6 cellobiohydrolases
Biochemical Journal, 2019Abstract Cellobiohydrolases (CBHs) from glycoside hydrolase family 6 (GH6) make up an important part of the secretome in many cellulolytic fungi. They are also of technical interest, particularly because they are part of the enzyme cocktails that are used for the industrial breakdown of lignocellulosic biomass.
Stefan Jarl Christensen +4 more
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Cellobiohydrolases Produce Different Oligosaccharides from Chitosan
Biomacromolecules, 2016Chito-oligosaccharides (COSs) are bioactive molecules with interesting characteristics; however, their exploitation is still restricted due to limited amounts accessible with current production strategies. Here we present a strategy for the production of COSs based on hydrolysis of chitosan by using readily available glycosidases.
Gregor, Tegl +6 more
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A bacterial GH6 cellobiohydrolase with a novel modular structure
Applied Microbiology and Biotechnology, 2017Cel6D from Paenibacillus barcinonensis is a modular cellobiohydrolase with a novel molecular architecture among glycosyl hydrolases of family 6. It contains an N-terminal catalytic domain (family 6 of glycosyl hydrolases (GH6)), followed by a fibronectin III-like domain repeat (Fn31,2) and a C-terminal family 3b cellulose-binding domain (CBM3b).
Liliana, Cerda-Mejía +4 more
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Cellobiohydrolases of Penicillium pinophilum
1988Publisher Summary This chapter describes the preparation and purification procedure Penicillium pinophilum cellulase. It describes the cultures of P. pinophilum that can be prepared by stationary or submerged culture techniques. Penicillium pinophilum cellulase contains two immunologically distinct cellobiohydrolases. These can be separated and
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Domain function in Trichoderma reesei cellobiohydrolases
Journal of Biotechnology, 1992The filamentous fungus, Trichoderma reesei produces all of the enzymatic activities required for efficient hydrolysis of highly ordered crystalline cellulose. All the principal enzymes involved in cellulose hydrolysis possess two functional domains, one directly involved in catalysis and the other in substrate recognition and binding.
Teeri, Tuula +4 more
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Engineering Thermostable Fungal Cellobiohydrolases
2013Meeting the world's growing energy demands while protecting our fragile environment is a challenging issue. Second generation biofuels are liquid fuels like long-chain alcohols produced from lignocellulosic biomass. To reduce the cost of biofuel production, we engineered fungal family 6 cellobiohydrolases (Cel6A) for enhanced thermostability using ...
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