Results 61 to 70 of about 6,444 (205)
Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose
Biotechnology for Biofuels, 2020 Background Molecular-scale mechanisms of the enzymatic breakdown of cellulosic biomass into fermentable sugars are still poorly understood, with a need for independent measurements of enzyme kinetic parameters.
Akshata R. Mudinoor +6 more
doaj +1 more source
Protein Science, Volume 35, Issue 9, September 2026.Abstract The design of proteins with desired biophysical properties, such as high solubility and low aggregation propensity, is crucial for various biotechnological and biomedical applications. While deep learning‐based methods like ProteinMPNN have shown remarkable success in protein sequence design, their direct output may not always exhibit optimal ...
Sacha T. Larda +2 more
wiley +1 more source
Liquid assays of endoglucanase, β-glucosidase, and cellobiohydrolase.
, 2014 Reducing sugars released from CMC (endoglucanase activity) and Avicel (β-glucosidase activity) when treated with fungal filter paper culture supernatant and p-nitrophenol release from pNPβG (cellobiohydrolase activity). Tests were performed in triplicate,
Hannah T. Reynolds (512674) +1 more
core +1 more source
Soil Science Society of America Journal, Volume 90, Issue 5, September/October 2026.Abstract Soil microbial biomass (MB) generally exhibits a narrower C:N than soil organic matter (SOM), creating a stoichiometric discrepancy hypothesized to be reconciled in part by C‐ and N‐acquiring enzyme activities. We used soils from an at‐equilibrium long‐term experiment to evaluate the degree to which MB C:N plasticity and enzyme activities ...
Tianze Song +5 more
wiley +1 more source
Hypocrea jecorina Cellobiohydrolase I Stabilizing Mutations Identified Using Noncontiguous Recombination [PDF]
, 2013 Noncontiguous recombination (NCR) is a method to identify pieces of structure that can be swapped among homologous proteins to create new, chimeric proteins. These “blocks” are encoded by elements of sequence that are not necessarily contiguous along
Bedbrook, Claire N. +3 more
core
Frontiers in Microbiology, 2017 The rumen bacterium Cellulosilyticum ruminicola H1 efficiently hydrolyzes cellulose. To gain insights into the regulatory mechanisms of cellulase synthesis, comparative transcriptome analysis was conducted for cultures grown on 2% filter paper, 0.5 and 0.
Shanzhen Li +7 more
doaj +1 more source
Soil Science Society of America Journal, Volume 90, Issue 5, September/October 2026.Abstract Crop rotational diversity is hypothesized to enhance soil organic carbon (SOC) surface stocks, yet the long‐term SOC response to maize (Zea mays L.)–soybean [Glycine max (L.) Merr.] (MS) relative to continuous maize (MM) is inconsistent despite the greater residue C inputs in MM.
Pei‐Chen Lee +4 more
wiley +1 more source
Effects of polystyrene nanoparticles on the microbiota and functional diversity of enzymes in soil
Environmental Sciences Europe, 2018 Background The increasing production of nanoplastics and the fragmentation of microplastics into smaller particles suggest a plausible yet unclear hazard in the natural environment, such as soil.
T. T. Awet +9 more
doaj +1 more source
Cropping system and nitrogen fertilization modulate soil enzyme kinetics
Soil Science Society of America Journal, Volume 90, Issue 5, September/October 2026.Abstract The decomposition of soil organic matter and nutrient cycling are largely mediated by extracellular enzymes, yet the effect of soil management on enzyme kinetic (Michaelis constant [Km] and maximum velocity [Vmax]) remains poorly understood.
A. A. Khaleel +2 more
wiley +1 more source
Scientific Reports, 2017 To realize the economical production of ethanol and other bio-based chemicals from lignocellulosic biomass by consolidated bioprocessing (CBP), various cellulases from different sources were tested to improve the level of cellulase secretion in the yeast
Cho-Ryong Lee +6 more
doaj +1 more source