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Analysis of Golgi Morphology Using Immunofluorescence and CellProfiler Software
2022The architecture of the Golgi apparatus in mammalian cells changes dynamically in response to internal and external cues and may be permanently altered in disease states. Here, we present a method to quantify changes in Golgi morphology using immunofluorescence and confocal microscopy followed by CellProfiler software analysis.
Isabel, Mejia +2 more
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CellProfiler and KNIME: Open-Source Tools for High-Content Screening
Methods in Molecular Biology, 2019High-content screening (HCS) has established itself in the world of the pharmaceutical industry as an essential tool for drug discovery and drug development. HCS is currently starting to enter the academic world and might become a widely used technology.
Marc Bickle +2 more
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CellProfiler Analyst: interactive data exploration, analysis and classification of large biological image sets. [PDF]
Bioinformatics, 2016 Abstract Summary: CellProfiler Analyst allows the exploration and visualization of image-based data, together with the classification of complex biological phenotypes, via an interactive user interface designed for biologists and data scientists.
Anne E Carpenter +2 more
exaly +3 more sources
Methods, 2022
Analytical pipeline, which is used for various analysis application, of CellProfiler, an open-source software for cell imaging analysis, is very important. In the present study, to examine whether intracellular proteins can be discriminated using a combination of CellProfiler and ImageJ, we analyzed neuroblastoma and monocytic cell lines, and disease ...
Hideyuki Okano, Kazuya Iwabuchi
exaly +3 more sources
Analytical pipeline, which is used for various analysis application, of CellProfiler, an open-source software for cell imaging analysis, is very important. In the present study, to examine whether intracellular proteins can be discriminated using a combination of CellProfiler and ImageJ, we analyzed neuroblastoma and monocytic cell lines, and disease ...
Hideyuki Okano, Kazuya Iwabuchi
exaly +3 more sources
Using CellProfiler for Automatic Identification and Measurement of Biological Objects in Images
Current Protocols in Molecular Biology, 2008AbstractVisual analysis is required to perform many biological experiments, from counting yeast colonies to measuring the size and shape of individual cells or the intensity of fluorescently labeled proteins within them. This unit outlines the use of CellProfiler, a free, open‐source image analysis tool that extracts quantitative information from ...
Martha S, Vokes, Anne E, Carpenter
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Generating realistic single-cell images from CellProfiler representations
Medical Image AnalysisHigh-throughput imaging techniques acquire large amounts of images efficiently. These images contain rich biological information including cellular processes. A common method to analyse them is to encode them into quantitative representation vectors. Generally, there are two ways to extract cell biological information into representations, hand-crafted
Yanni Ji +3 more
openaire +2 more sources