Results 101 to 110 of about 4,741,341 (392)

Physico-electrochemical Characterization of Pluripotent Stem Cells during Self-Renewal or Differentiation by a Multi-modal Monitoring System. [PDF]

, 2017
Monitoring pluripotent stem cell behaviors (self-renewal and differentiation to specific lineages/phenotypes) is critical for a fundamental understanding of stem cell biology and their translational applications.
Horner, Christopher B, Low, Karen, Maldonado, Maricela, Manjunath, Chetas, Myung, Nosang V, Nam, Jin, Perez, Mark, Wong, Lauren Y   +7 more
core   +3 more sources

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

Hierarchical folding and reorganization of chromosomes are linked to transcriptional changes in cellular differentiation

Molecular Systems Biology, 2015
Mammalian chromosomes fold into arrays of megabase‐sized topologically associating domains (TADs), which are arranged into compartments spanning multiple megabases of genomic DNA.
J. Fraser, Carmelo Ferrai, A. Chiariello, M. Schueler, T. Rito, Giovanni Laudanno, Mariano Barbieri, Benjamin L. Moore, Dorothee C. A. Kraemer, Stuart Aitken, S. Q. Xie, Kelly J. Morris, M. Itoh, H. Kawaji, Ines Jaeger, Y. Hayashizaki, Piero Carninci, A. Forrest, C. Semple, J. Dostie, A. Pombo, M. Nicodemi   +21 more
semanticscholar   +1 more source

Proteomic analyses reveal misregulation of LIN28 expression and delayed timing of glial differentiation in human iPS cells with MECP2 loss-of-function. [PDF]

, 2019
Rett syndrome (RTT) is a pervasive developmental disorder caused by mutations in MECP2. Complete loss of MECP2 function in males causes congenital encephalopathy, neurodevelopmental arrest, and early lethality.
Carromeu, Cassiano, Freitas, Beatriz CG, Ghosh, Anirvan, Hu, Xindao, Kim, Jean J, Lavallée-Adam, Mathieu, Miller, Meghan T, Muotri, Alysson R, Savas, Jeffrey N, Yates, John R   +9 more
core   +2 more sources

Chromatin conformation signatures of cellular differentiation [PDF]

Genome Biology, 2009
AbstractOne of the major genomics challenges is to better understand how correct gene expression is orchestrated. Recent studies have shown how spatial chromatin organization is critical in the regulation of gene expression. Here, we developed a suite of computer programs to identify chromatin conformation signatures with 5C technology http://Dostielab.
Mathieu Rousseau, Mathieu Blanchette, Josée Dostie, Solomon Shenker, Maria Ferraiuolo, Yoshihide Hayashizaki, James Fraser   +6 more
openaire   +2 more sources

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

R-loops regulate promoter-proximal chromatin architecture and cellular differentiation

Nature Structural &Molecular Biology, 2015
Numerous chromatin-remodeling factors are regulated by interactions with RNA, although the contexts and functions of RNA binding are poorly understood.
Poshen B. Chen, Hsiuyi V. Chen, Diwash Acharya, O. Rando, T. Fazzio   +4 more
semanticscholar   +1 more source

Dynamic shifts in chromatin states differentially mark the proliferative basal cells and terminally differentiated cells of the developing epidermis

Epigenetics, 2020
Post-translational modifications on nucleosomal histones represent a key epigenetic regulatory mechanism to mediate the complex gene expression, DNA replication, and cell cycle changes that occur in embryonic cells undergoing lineage specification ...
Yan Ting Shue, Kang Ting Lee, Benjamin William Walters, Hui Binn Ong, Shaktheeshwari Silvaraju, Wei Jun Lam, Chin Yan Lim   +6 more
doaj   +1 more source

Rigidity of silicone substrates controls cell spreading and stem cell differentiation. [PDF]

, 2016
The dependences of spreading and differentiation of stem cells plated on hydrogel and silicone gel substrates on the rigidity and porosity of the substrates have recently been a subject of some controversy.
Groisman, Alex, Gutierrez, Edgar, Lee, Sin-Ae, Ronan, Edward, Tkachenko, Eugene, Vertelov, Grigory   +5 more
core   +2 more sources

CELLULAR DIFFERENTIATION IN THE THYMUS [PDF]

The Journal of Experimental Medicine, 1970
Young adult rat thymus and lymph node cell subpopulations were obtained by differential flotation on discontinuous BSA density gradients and assayed for properties characteristic of mature thymus-derived lymphocytes. One such subpopulation (C) of thymocytes was enriched in its ability to respond mitotically to a hemiallogeneic MLR stimulus, to localize
Byron H. Waksman, D. G. Colley, A. Y. Shih Wu   +2 more
openaire   +2 more sources