Results 41 to 50 of about 3,742,959 (293)

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

open access: yesFEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

open access: yesFEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli   +3 more
wiley   +1 more source

Centrifugal model tests on behavior of soil and tunnel under underground water leakage

open access: yesYantu gongcheng xuebao, 2023
Underground water leakage may lead to pavement settlement, threatening the safety of surrounding soil and adjacent structures. The centrifugal model tests are conducted to explore the response of soil and tunnel under the underground water leakage.
TIAN Guyu , LIU Sujia , ZHANG Ga
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Research on evaluation of Self-weight collapsibility for large-thickness collapsible loess using centrifugal model test

open access: yes工程科学与技术, 2016
:Ili loess, a typically large-thickness collapsible eolian loess, has parameters that uniformly changes along the depth direction. According to this characteristic and engineering requirements, centrifugal model tests of the self-weight collapsible loess,
An Peng, XING Yi-chuan
doaj  

Time-Marching Throughflow Analysis of Centrifugal Compressors with Boundary Conditions Based on Newton’s Method

open access: yesApplied Sciences, 2022
The meridional distribution of the flow parameters inside the centrifugal compressor is of great importance to its overall performance, as well as its matching performance under a thermal cycle.
Chen Yang   +5 more
doaj   +1 more source

Centrifuge model testing for pile foundation reuse [PDF]

open access: yesInternational Journal of Physical Modelling in Geotechnics, 2011
With continuous development in the urban environment, the ground is becoming increasingly congested with redundant foundations. The underground development of services and infrastructure already restricts the location of new building foundations and the redundant foundations only add to this problem.
Begaj, L., McNamara, A.M.
openaire   +2 more sources

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

open access: yesFEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan   +2 more
wiley   +1 more source

Development and performance testing of centrifugal model test equipment for frozen soil

open access: yesYantu gongcheng xuebao
The centrifugal model test is an effective method to solve the problem of frozen soil engineering in a long time span. But the refrigeration end of the existing centrifugal model test devices can only control the temperature boundary, and can not form a ...
ZHOU Jie 1, 2, GUO Zhongqiu 1, SHI Zhenming 1, 2, LIU Chengjun 1, ZHOU Huade 1, BAN Chao 1
doaj   +1 more source

A centrifugal model test of microbial carbonate precipitation as liquefaction countermeasure

open access: yes, 2011
社会資本の維持管理や温室効果ガス排出抑制が課題となる中,地盤改良技術についても,既設構造物の補修や補強に適用可能で,温室効果ガスの排出が少ない技術が求められる。微生物代謝で発生する二酸化炭素と土の間隙中のカルシウム源から炭酸カルシウムを析出させて土を固化する炭酸カルシウム法は,既設構造物直下の地盤に適用しやすく,改良材製造時の二酸化炭素排出が少ない技術として期待される。本研究では,炭酸カルシウム法により豊浦砂を固化し,三軸圧縮試験および動的遠心模型実験を行った。三軸圧縮試験では ...
Y. Inagaki   +5 more
semanticscholar   +1 more source

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