Results 161 to 170 of about 240,760 (305)

A Fully Human Engineered Bone Niche With Endogenous Osteoclastogenesis Reveals Osteoclast‐Dependent Osteomimicry in Prostate Cancer Cells

Advanced Healthcare Materials, EarlyView.
A human 3D bone niche integrating osteoblasts and osteoclasts enables co‐culture with prostate cancer cell lines and patient‐derived organoids. The engineered niche (i) models aspects of reciprocal phenotypic regulation between osteoblasts and cancer cells, (ii) captures osteoclast‐enhanced osteomimicry in tumor cells, and (iii) establishes a ...
Andrea Mazzoleni, Robin Dolgos, Thomas Menter, Boris Dasen, Arnaud Scherberich, Clémentine Le Magnen, Manuele G. Muraro, Ivan Martin   +7 more
wiley   +1 more source

Efficient lentivirus concentration using a tabletop centrifuge. [PDF]

Mol Biol Rep
Müller E, Boesner J, Karaköse Z, Schwarzer R, Schwarzer-Sperber HS.   +4 more
europepmc   +1 more source

Separation of Penaeus vannamei haemocyte subpopulations by iodixanol density gradient centrifugation

, 2013
Methodologies for separation of immune cell subpopulations are essential tools in immunology studies. Up to date, only one methodology for separating crustacean haemocyte subpopulations using Percoll density gradient centrifugation has been described.In ...
Grauwet, K., Dantas-Lima, J.J., An, N.T.T., Sorgeloos, P., Corteel, M., Tuan, V.V., Nauwynck, H.J.   +6 more
core  

A Systematic Study of GelMA‐Carbopol Bioinks for High‐Fidelity Extrusion 3D Bioprinting at Physiological Temperatures

Advanced Healthcare Materials, EarlyView.
Gonzalez Martinez and collaborators develop a strategy to formulate high performance GelMA‐based bioinks with low solids contents. The resulting bioinks enable 3D bioprinting at 37 °C of high‐fidelity structures with tunable mechanical properties that support high cell viability and function.
David A. González‐Martínez, Aidee V. Arizpe Tafoya, Eduardo González‐Martínez, Mabel Barreiro Carpio, Laura Daniela Serrano Andrade, Christopher T. N. Chow, Mohammadhossein Dabaghi, Jeremy A. Hirota, Jose M. Moran‐Mirabal   +8 more
wiley   +1 more source

Protocol to isolate endosomal and small extracellular vesicles from cultured cells through ultracentrifugation. [PDF]

STAR Protoc
Domingues MN, Palhinhas L, Pereira P, Ferreira JV.   +3 more
europepmc   +1 more source

Tracking Spatiotemporal Extracellular Matrix Evolution and Tissue Fusion in 3D Microtissues via Click Chemistry‐Based Metabolic Labelling

Advanced Healthcare Materials, EarlyView.
Metabolic labeling of nascent proteins in 3D microtissue spheroids provides a powerful analytical approach for large‐scale tissue engineering. Incorporation of non‐canonical amino acids with fluorescent tagging enables spatiotemporal investigation of extracellular matrix deposition and its evolution during multicellular tissue development and fusion ...
Theresa Koenig, F. Max Yavitt, Laura Veenendaal, Tim B. F. Woodfield   +3 more
wiley   +1 more source

Microgel‐Based 3D Bioprinting: A Convergent Strategy Integrating Material Design, Jamming Dynamics, and Biological Function

Advanced Healthcare Materials, EarlyView.
Microgel‐based 3D printed constructs represent a compelling and versatile innovation for engineering architecturally complex, dynamically remodelable, and biocompatible structures with high structural fidelity and bioactivity. By integrating material design, biofabrication, and biological function, these systems enable the development of adaptive ...
Elena Ghighină, Andreea Ioana Dinu, Jana Ghițman   +2 more
wiley   +1 more source

Protocol for the biochemical isolation of endosomal membranes associated with endoplasmic reticulum in HeLa cells. [PDF]

STAR Protoc
Da Graça J, Morel E.
europepmc   +1 more source

Neutrophil‐Mimetic MRI Enables Ultra‐Early Detection of Vascular Inflammation After Stroke

Advanced Healthcare Materials, EarlyView.
In this study, we developed neutrophil‐mimetic MRI probes that detect ultra‐early neuroinflammation following ischemic stroke by targeting E‐selectin. Within seconds of their injection, these microparticles mimic initial leukocyte adhesion to the activated cerebral endothelium.
Marion Isabelle Morvan, Damien Levard, Eloïse Lemarchand, Amal Boublay, Charly Helaine, Jolan Malherbe, Mikaël Naveau, Didier Goux, Igor Khalin, Ankita Talukdar, Cheun Pelleter, Charlène Jacqmarcq, Erwan Baudron, Isabelle Bardou, Sara Martinez de Lizarrondo, Maxime Gauberti, Denis Vivien, Antoine Philippe Fournier   +17 more
wiley   +1 more source

Bacterial Extracellular Vesicles (BEVs) Derived from Chryseobacterium Inhibit Dengue Virus Infection by Disrupting Its Structural Integrity. [PDF]

J Extracell Vesicles
Gao Y, Zhang L, Zhang T, Yao Q, Gao R, Wang Y, Zhao Y, Zhou T, Chen J, Zhang X, Ren H, Zhu Y, Zhao P, Qi Z, Luo L, Qin Z.   +15 more
europepmc   +1 more source