Results 1 to 10 of about 236,440 (248)

Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90% [PDF]

MethodsX, 2021
Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa, Andrea Galuppo, Rômulo Batista Rodrigues, Nathalia dos Santos Teixeira, Thaiza Rodrigues de Freitas, Thales de Souza França, Lis Santos Marques, Itamar Cossina Gomes, Danilo Pedro Streit, Jr.   +8 more
doaj   +4 more sources

Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]

Reproductive Medicine and Biology
Purpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano, Satoko Oigawa, Koichiro Ichizawa, Yukiko Tokuda, Mami Unagami, Mami Sekiguchi, Mayuko Furui, Kentaro Nakaoka, Ayumu Ito, Rika Hayashi, Yuko Tamaki, Yuko Hayashi, Yusuke Fukuda, Yukiko Katagiri, Masahiko Nakata, Koichi Nagao   +15 more
doaj   +3 more sources

Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia [PDF]

Life, 2021
In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler, Sureyya Melil, Umit Ozekici, Yaprak Donmez Cakil, Belgin Selam, Mehmet Cincik   +5 more
doaj   +4 more sources

Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells [PDF]

Bioengineering, 2017
Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to ...
Yuxi Sun, Palaniappan Sethu
doaj   +4 more sources

Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures [PDF]

MethodsX, 2023
Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay, Rashmi Ravindran Nair, Deepti Sharan, Kishor Jakkala, Parthasarathi Ajitkumar   +4 more
doaj   +2 more sources

Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation [PDF]

BMC Research Notes, 2023
Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza, Anyonya R. Guntur, Calvin P. Vary, Carlos A. Gartner, Madeleine Nowak, Robert A. Koza   +5 more
doaj   +2 more sources

Mass Cytometry Reveals Comparable Proportions of Leukocyte Subsets and Cell Surface P2X7 or CD39 on Human Peripheral Blood Mononuclear Cell Samples Isolated by Ficoll-Paque or SepMate Tube Density Gradient Centrifugation [PDF]

Cells
P2X7 is an adenosine 5′-triphosphate (ATP)-gated ion channel. CD39 (ectonucleotidase triphosphate diphosphohydrolase-1) hydrolyses ATP to reduce its extracellular concentration to limit P2X7 activation.
Amal Elhage, Bavani Gunasegaran, Natalie Smith, Ross J. Turner, Debbie Watson, Helen M. McGuire, Ronald Sluyter   +6 more
doaj   +2 more sources

Comparison of Sperm Telomere Length between Two Sperm Selection Procedures: Density Gradient Centrifugation and Zeta Potential [PDF]

International Journal of Fertility and Sterility, 2020
Background Telomeres are particular sequences of DNA located at the end of the eukaryotic chromosomes that are essential for genome integrity. Telomere length in spermatozoa differs among males, as well as spermatozoa.
Razieh Ghorbani-sini, Tayebeh Izadi, Marziyeh Tavalaee, Leila Azadi, Mehdi Hajian, Mahshid Rahimi Zamani, Mohammad Hossein Nasr-Esfahani   +6 more
doaj   +2 more sources

The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa [PDF]

BMC Veterinary Research, 2022
Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls.
Maria Eberhardt, Sylwia Prochowska, Anna M. Duszewska, Ann Van Soom, Wanda Olech, Wojciech Niżański   +5 more
doaj   +2 more sources

Comparative Efficacy of Swim-Up, Density-Gradient Centrifugation, and Microfluidic Sorting in Sperm Preparation, and the Impact on Motility, Morphology, and DNA Integrity [PDF]

International Journal of General Medicine
Zi-Na Wen, Li Duan, Yong Chen, Qing-Hong Qiu, Gang Liu, Ning Luo, Peng-Hao Li, Er-Po Tian, Ren-Shan Ge Andrology Laboratory, Sichuan Jinxin Xinan Women and Children Hospital, Chengdu, Sichuan, People’s Republic of ChinaCorrespondence: Ren-Shan Ge ...
Wen ZN, Duan L, Chen Y, Qiu QH, Liu G, Luo N, Li PH, Tian EP, Ge RS   +8 more
doaj   +2 more sources