Swim‐up method is superior to density gradient centrifugation for preserving sperm DNA integrity during sperm processing [PDF]
Reproductive Medicine and BiologyPurpose This study aimed to evaluate the effects of swim‐up and density gradient centrifugation methods on sperm DNA fragmentation. Methods Nineteen normozoospermic patient samples with ≥100 × 106 motile sperms were included in this study.
Kenji Amano +15 more
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Sperm selection of cryopreserved milt of catfish (Rhamdia quelen) by density gradient centrifugation with AllGrad® 90% [PDF]
MethodsX, 2021 Density gradient centrifugation is a technique used to wash or separate samples of cryopreserved milt, mainly in humans and bovines allowing, for example, reducing the concentration of cryoprotectants or choosing the best portion of sperm.
Maritza Pérez-Atehortúa +8 more
doaj +4 more sources
Sperm Selection and Embryo Development: A Comparison of the Density Gradient Centrifugation and Microfluidic Chip Sperm Preparation Methods in Patients with Astheno-Teratozoospermia [PDF]
Life, 2021 In recent years, microfluidic chip-based sperm sorting has emerged as an alternative tool to centrifugation-based conventional techniques for in vitro fertilization.
Cagla Guler +5 more
doaj +4 more sources
The influence of Percoll® density gradient centrifugation before cryopreservation on the quality of frozen wisent (Bison bonasus) epididymal spermatozoa [PDF]
BMC Veterinary Research, 2022 Background The wisent (Bison bonasus) is a species that has undergone a population bottleneck. Homozygosity is prevalent within the population and may have a negative impact on semen quality in wisent bulls.
Maria Eberhardt +5 more
doaj +3 more sources
Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation [PDF]
Nanomaterials, 2016 Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors.
Monika Mortimer +3 more
doaj +4 more sources
Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation. [PDF]
Curr Protoc Cell Biol, 2016 Lipid droplets are organelles found in most mammalian cells, as well as various plant tissues and yeast. They are composed of a core of neutral lipids surrounded by a membrane monolayer of phospholipids and cholesterol into which specific proteins are ...
Brasaemle DL, Wolins NE.
europepmc +3 more sources
Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells [PDF]
Bioengineering, 2017 Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to ...
Yuxi Sun, Palaniappan Sethu
doaj +3 more sources
Percoll discontinuous density gradient centrifugation method for the fractionation of the subpopulations of Mycobacterium smegmatis and Mycobacterium tuberculosis from in vitro cultures [PDF]
MethodsX, 2023 Bacterial populations in the in vitro laboratory cultures, environment, and patients contain metabolically different subpopulations that respond differently to stress agents, including antibiotics, and emerge as stress tolerant or resistant strains.
Srinivasan Vijay +4 more
doaj +2 more sources
Purification of functional mouse skeletal muscle mitochondria using percoll density gradient centrifugation [PDF]
BMC Research Notes, 2023 Objective Our goal was to isolate purified mitochondria from mouse skeletal muscle using a Percoll density gradient and to assess bioenergetic function and purity via Seahorse Extracellular Flux (XF) Analyses and mass spectrometry.
Rea Victoria P. Anunciado-Koza +5 more
doaj +2 more sources
Mass Cytometry Reveals Comparable Proportions of Leukocyte Subsets and Cell Surface P2X7 or CD39 on Human Peripheral Blood Mononuclear Cell Samples Isolated by Ficoll-Paque or SepMate Tube Density Gradient Centrifugation [PDF]
CellsP2X7 is an adenosine 5′-triphosphate (ATP)-gated ion channel. CD39 (ectonucleotidase triphosphate diphosphohydrolase-1) hydrolyses ATP to reduce its extracellular concentration to limit P2X7 activation.
Amal Elhage +6 more
doaj +2 more sources