Density Gradient Centrifugation-Independent Purification of Human Basophils. [PDF]
Curr ProtocAbstractBasophils represent the rarest type of granulocyte in human peripheral blood. Thus, researching basophils has historically been challenging and has often been reliant on enrichment protocols using density gradient centrifugation. This article describes a novel, fast, and cost‐effective method to purify highly viable human basophils from ...
Gray N +4 more
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Separation of Bacteria, Protozoa and Carbon Nanotubes by Density Gradient Centrifugation [PDF]
Nanomaterials, 2016 Sustainable production and use of carbon nanotube (CNT)-enabled materials require efficient assessment of CNT environmental hazards, including the potential for CNT bioaccumulation and biomagnification in environmental receptors.
Monika Mortimer +3 more
doaj +2 more sources
New device for sperm preparation involving migration-gravity sedimentation without centrifugation compared with density-gradient centrifugation for normozoospermic intrauterine insemination [PDF]
F&S Reports, 2020 Objective: To investigate the efficacy of a new device for sperm preparation involving migration-gravity sedimentation without centrifugation (MIGLIS), compared with density-gradient centrifugation (DGC) for normozoospermic intrauterine insemination (IUI)
Kenichi Tatsumi, M.D., Ph.D. +4 more
doaj +2 more sources
Microfluidic Adaptation of Density-Gradient Centrifugation for Isolation of Particles and Cells [PDF]
Bioengineering, 2017 Density-gradient centrifugation is a label-free approach that has been extensively used for cell separations. Though elegant, this process is time-consuming (>30 min), subjects cells to high levels of stress (>350 g) and relies on user skill to ...
Yuxi Sun, Palaniappan Sethu
doaj +2 more sources
Helper-free Production of Laboratory Grade AAV and Purification by Iodixanol Density Gradient Centrifugation [PDF]
Molecular Therapy: Methods & Clinical Development, 2018 Adeno-associated virus (AAV) is one of the most promising gene therapy vectors and is widely used as a gene delivery vehicle for basic research. As AAV continues to become the vector of choice, it is increasingly important for new researchers to have ...
Sean M. Crosson +3 more
doaj +2 more sources
Separation of Stabilized MOPS Gold Nanostars by Density Gradient Centrifugation [PDF]
ACS Omega, 2017 Kavita Chandra +3 more
doaj +2 more sources
Fertility & Reproduction, 2023 Background and Aims: While density gradient centrifugation (DGC) is a conventional method in sperm preparation, this method can induce reactive oxygen species (ROS) which cause sperm dysfunction, sperm DNA damage, a recent advancement in sperm processing
Nguyet Minh Ho +3 more
doaj +1 more source
The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring [PDF]
Veterinary World, 2022 Background and Aim: The production of male calf beef cattle is an agricultural innovation needed to increase the farm's productivity as a provider of meat sources.
Dewa Ketut Meles +7 more
doaj +1 more source
Life, 2022 The biological variability of semen and sperm DNA fragmentation (SDF) parameters in a longitudinal intrauterine insemination (IUI) trial over multiple IUI cycles was investigated.
Usha Punjabi +5 more
doaj +1 more source
Density gradient centrifugation compromises bone marrow mononuclear cell yield. [PDF]
PLoS ONE, 2012 Bone marrow mononuclear cells (BMNCs) are widely used in regenerative medicine, but recent data suggests that the isolation of BMNCs by commonly used Ficoll-Paque density gradient centrifugation (DGC) causes significant cell loss and influences graft ...
Claudia Pösel +5 more
doaj +1 more source