Asian Pacific Journal of Tropical Biomedicine, 2014 Objective: To obtain the bone marrow mesenchymal stem cells (BMSCs), complete phenotypic identification and successfully transfect rat BMSCs by recombinant plasmid pEGFP/Ang-1.
Xiu-Qun Zhang +3 more
doaj +1 more source
Distinct Intracellular Compartments Involved in Invariant Chain Degradation and Antigenic Peptide Loading of Major Histocompatibility Complex (MHC) Class II Molecules [PDF]
, 1997 Major histocompatibility complex (MHC) class II molecules are transported to intracellular MHC class II compartments via a transient association with the invariant chain (Ii).
Ferrari, G. +3 more
core +1 more source
Motility fractionation of bacteria by centrifugation
, 2013 Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To
Di Leonardo, Roberto +4 more
core +1 more source
Minimal Information About Sample Preparation for Phosphoproteomics [PDF]
, 2009 This guideline describes parameters and conditions involved in phosphopeptide sample preparation. It covers from the description and preparation of the cells and tissues to the fractionation and specific enrichment of phosphopeptides for MS analysis. The
David Ovelleiro +3 more
core +2 more sources
Journal of Men's Health, 2022 Background: To evaluate the efficiency of bacterial clearance of different sperm preparation procedure. Methods: A total of 46 semen samples were collected in this study, each semen sample was divided into four equal parts (0.5 mL each).
Zhiheng Chen +6 more
doaj +1 more source
Sorting and transmission electron microscopy analysis of single or double wall carbon nanotubes [PDF]
, 2009 On the basis of the recent progress on the sorting of carbon nanotubes’ structure with respect to their diameter or number of walls, we investigate by transmission electron microscopy the sorting efficiency, with a comparison with optical absorption ...
Arnold +14 more
core +2 more sources
Timing of semen cryopreservation: before or after processing? [PDF]
Revista Brasileira de Ginecologia e ObstetríciaObjective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with
Ana Paula de Souza Kussler +4 more
doaj +1 more source
Nuclear translocation and signalling of L1-CAM in human carcinoma cells requires ADAM10 and presenilin/gamma-secretase activity [PDF]
, 2009 L1-CAM (L1 cell-adhesion molecule), or more simply L1, plays an important role in the progression of human carcinoma. Overexpression promotes tumour-cell invasion and motility, growth in nude mice and tumour metastasis.
Altevogt, Peter +6 more
core +2 more sources
Comparative proteomic analysis of spermatozoa isolated by swim-up or density gradient centrifugation [PDF]
, 2015 BACKGROUND: Reports about the morphologic and functional characteristics of spermatozoa prepared by density gradient centrifugation (DC) or swim-up (SU) have produced discordant results.
A Ashrafzadeh +56 more
core +2 more sources
Novel Bacterial Diversity and Fragmented eDNA Identified in Hyperbiofilm-Forming Pseudomonas aeruginosa Rugose Small Colony Variant [PDF]
, 2020 Pseudomonas aeruginosa biofilms represent a major threat to health care. Rugose small colony variants (RSCV) of P. aeruginosa, isolated from chronic infections, display hyperbiofilm phenotype.
Deng, Binbin +10 more
core +1 more source