Results 61 to 70 of about 3,338,865 (284)
Red blood cell layers after Percoll density gradient centrifugation.
, 2015 Seven RBC fractions were generated by Percoll density gradient centrifugation. Least dense fraction on the top consisted of youngest RBC; increasing density indicated increasing RBC age.
Daniel Alexander Bizjak (728303) +3 more
core +1 more source
Molecular Oncology, EarlyView.Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla +9 more
wiley +1 more source
Spira mirabilis enhanced density gradient centrifguation [PDF]
, 2013 The centrifugal microfluidic “lab-on-a-disc” paradigm is particularly suited to the pre-conditioning of whole blood. In this work we define the geometry of sedimentation chambers using the Spira Mirabilis (equiangular spiral) and so enhance the ...
Jens Ducree +5 more
core +2 more sources
Bio-Protocol, 2013 Peripheral blood mononuclear cells (PBMCs) are chiefly lymphocytes and monocytes. PBMCs are separated from the whole blood by a density gradient centrifugation method using Ficoll Histopaque.
Santosh Panda, Balachandran Ravindran
doaj +1 more source
Purification of the Scrapie Agent by Density Gradient Centrifugation
Journal of General Virology, 1984 Plasma membrane-enriched preparations from scrapie-infected and healthy hamster brains, as well as preparations of neural retina, were sonicated, then separated by rate-zonal sedimentation in 10 to 25% Nycodenz gradients. Gradient fractions were extracted with 0.5% Triton X-100 and re-fractionated by equilibrium density centrifugation in linear 25 to ...
R F, Marsh +4 more
openaire +2 more sources
Finding novel vulnerabilities of hypomorphic BRCA1 alleles
Molecular Oncology, EarlyView.Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder +10 more
wiley +1 more source
Effect of Density Gradient Centrifugation on Quality and Recovery Rate of Equine Sperm [PDF]
, 2009 Density gradient centrifugation of sperm is a common assisted-reproduction procedure in humans used to improve semen quality. The technique allows sperm separation based on their isopycnic points.
Edmond, Ann J.
core +1 more source
Isolation and Culture of Murine Hepatic Stellate Cells
Bio-Protocol, 2019 Hepatic stellate cells (HSCs), alternatively known as liver pericytes, can differentiate into myofibroblasts and secrete extra-cellular matrix components, thereby promoting wound healing and fibrosis.
Rucha Modak, Dietmar Zaiss
doaj +1 more source
Molecular Oncology, EarlyView.The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka +9 more
wiley +1 more source
Loss of proton‐sensing TDAG8 increases tumor progression in mouse models of colon cancer
Molecular Oncology, EarlyView.Loss of the pH‐sensing receptor TDAG8 accelerates colorectal cancer progression in mice. Animals lacking TDAG8 expression had increased tumor growth, DNA damage, and recruitment of tumor‐associated immune cells, including macrophages, neutrophils, and monocytes.
Ermanno Malagola +11 more
wiley +1 more source