Results 81 to 90 of about 239,867 (289)

Circular DNA's from HeLa cell nuclei and mitochondria

, 1970
An electron microscopic observation was made on the DNA's extracted from purified HeLa cell nuclei, mitochondria, and the whole cell, and fractionated by ethidium bromide-cesium chloride density gradient method or sucrose density gradient method. Nuclear
Oda, Takuzo, Yamamoto, Shinichiro, Hirata, Seiichi, Nishida, Shigeru, Omura, Sachiko   +4 more
core   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

A laboratory modification to testicular sperm preparation technique improves spermatogenic cell yield

Asian Journal of Andrology, 2014
Testicular sperm extraction is a common procedure used to find spermatogenic cells in men with nonobstructive azoospermia. The laboratory processing of biopsied testicular tissues needs to be performed meticulously to acquire a high yield of cells.
Sinan Ozkavukcu, Ebru Ibis, Sule Kizil, Suheyla Isbacar, Kaan Aydos   +4 more
doaj   +1 more source

Detection of circulating tumor cells in blood using an optimized density gradient centrifugation.

, 2003
The aim of the study was to compare the new density gradient centrifugation system OncoQuick with the standard density gradient centrifugation system Ficoll for improved tumor cell enrichment in blood of tumor patients. Evaluation of OncoQuick and Ficoll
Gertler, R;Rosenberg, R;Fuehrer, K;Dahm, M;Nekarda, H;Siewert, JR
core  

Purification of white spot syndrome virus by iodixanol density gradient centrifugation

, 2013
Up to now, only a few brief procedures for purifying white spot syndrome virus (WSSV) have been described. They were mainly based on sucrose, NaBr and CsCl density gradient centrifugation.
Corteel, Mathias, Pereira Dantas Da Rocha Lima, João José, Nauwynck, Hans, Cornelissen, Maria, Sorgeloos, Patrick, Bossier, Peter   +5 more
core   +1 more source

Purification of the Scrapie Agent by Density Gradient Centrifugation

Journal of General Virology, 1984
Plasma membrane-enriched preparations from scrapie-infected and healthy hamster brains, as well as preparations of neural retina, were sonicated, then separated by rate-zonal sedimentation in 10 to 25% Nycodenz gradients. Gradient fractions were extracted with 0.5% Triton X-100 and re-fractionated by equilibrium density centrifugation in linear 25 to ...
R F, Marsh, C, Dees, B E, Castle, W F, Wade, T L, German   +4 more
openaire   +2 more sources

A novel quinazolinone insulin receptor inhibitor and its synergy with an EGFR inhibitor in glucose‐driven glioblastoma

Molecular Oncology, EarlyView.
The novel styrylquinazolinone‐based molecule W1B effectively suppresses glioblastoma by inhibiting IGF1R and EGFR. In high‐glucose microenvironments driving tumor resistance, W1B acts synergistically with the EGFR inhibitor dacomitinib. This combination safely blocks compensatory survival signaling in zebrafish xenograft models. Showcasing promising in
Patryk Rurka, Wioleta Cieślik, Wojciech Płaziński, Katarzyna Stępnik, Anna Boguszewska‐Czubara, Elżbieta Kot, Robert Musioł, Mateusz Jasica, Anna Mrozek‐Wilczkiewicz, Katarzyna Malarz   +9 more
wiley   +1 more source

Improved progressive motility recovery with a standardized gradient-based approach for semen preparation in IUI

Reproductive Biology and Endocrinology
Objective To evaluate the laboratory performance of a standardized gradient-based sperm preparation system designed to improve progressive motility recovery during density gradient centrifugation for intrauterine insemination (IUI). Design Retrospective,
Felix Roth, Felix Haeberlin
doaj   +1 more source

Density-Gradient Centrifugation: Non-Ideal Sedimentation and the Interaction of Major and Minor Components

, 1965
A small amount of southern bean mosaic virus was contained in a narrow zone after density-gradient centrifugation, but in a much wider zone after centrifugation with a large amount of a second virus.
Brakke, Myron K, Daly, J. M.
core  

Zonal density gradient electrophoresis of intracellular membranes of brain cortex [PDF]

, 1969
Rat cerebral cortex was homogenized in a sucrose-free medium and a number of intracellular membrane fractions were isolated therefrom by means of differential and density-gradient centrifugation.
Sellinger, Otto Z., Borens, Robert N.
core   +1 more source