Preparation of Crude Subcellular Fractions by Differential Centrifugation
The employment of differential centrifugation to prepare crude fractions of subcellular particles from homogenates is often a necessary first step to a subsequent purification of one or more particles on a density gradient.
John Graham
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The process of infection with bacteriophage phiX174, XXX. Replication of double-stranded phiX DNA [PDF]
Intermediates involved in the replication of double-stranded phiX174 RF DNA have been identified and partially characterized. Analysis of pulse-labeled RF DNA suggests that the synthesis of progeny RF molecules involves, in part, the addition of ...
Knippers, Rolf +2 more
core
Two-fluid model for the simultaneous flow of colloids and fluids in porous media [PDF]
To describe the velocities of particles such as ions, protein molecules and colloids dispersed or dissolved in a fluid, it is important to also describe the forces acting on the fluid, including pressure gradients and friction of the fluid with the ...
Biesheuvel, P.M.
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Density separation of quiescent yeast using iodixanol
As yeast are starved of nutrients, they enter G0, a quiescent state. Quiescent yeast (Q) cells retain viability for extended periods of time and resume growth following supplementation of missing nutrients.
Ishtiaque Quasem +3 more
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Protease effects on the structure of acetylcholine receptor membranes from Torpedo californica. [PDF]
Protease digestion of acetylcholine receptor-rich membranes derived from Torpedo californica electroplaques by homogenization and isopycnic centrifugation results in degradation of all receptor subunits without any significant effect on the appearance in
Heuser, JE, Klymkowsky, MW, Stroud, RM
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Translational control by the multi-KH domain protein Scp160 [PDF]
The control of mRNA translation mediated by RNA-binding proteins (RBPs) is a key player in modulating gene expression. In S. cerevisiae, the multi-KH domain protein Scp160 associates with a large number of mRNAs and is present on membrane-bound and, to a
Schreck, Heidrun
core
The purpose of this experiment was to characterize the high density lipoproteins (HDL) as a function of hydrated density. HDL was subfractionated on the basis of hydrated density by CsCl density gradient centrifugation of whole serum or the d 1.063-1.25 ...
M C Cheung, J J Albers
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Formation of Self-Generated Gradients of Iodixanol
The formation of self-generated gradients of iodixanol from a solution of uniform concentration requires the use of vertical or near-vertical rotors.
John Graham
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Comparative sperm recovery rate after density gradient centrifugation with two media for in vitro fertilization. [PDF]
Fernandes NS +6 more
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Protocol to dissociate and isolate wide-diversity single cells by density gradient centrifugation from human hepatoblastoma tissue. [PDF]
Zhou X +6 more
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